بررسی مولکولی توکسوپلاسما در نمونه های جفتی سقط شده به روش pcr در اردبیل

Title

MOLECULAR STUDY OF TOXOPLASMOSIS IN ABORTED PLACENTA SAMPLES WITH NPCR METHOD IN ARDEBIL- IRAN

English Abstract

Toxoplasma gondii is a protozoan parasite that infects up to a third of the world’s population.Infection is mainly acquired by ingestion of food or water that is contaminated with oocysts shed by cats or by eating undercooked or raw meat containing tissue cysts. Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission to the fetus. Although Toxoplasma gondii infection generally shows subclinical courses in adults, it may have severe pathologic effects on the fetus. Infection in pregnant women may lead to abortion, stillbirth or other serious consequences in newborns. This study was conducted to test the utility of a polymerase chain reaction (PCR) assay to detect recent infections with Toxoplasma in abortive women. Two hundred placentas from aborted women in various gestational ages and with a history of abortion, admitted in Alavi Obstetric and Gynecological department of Ardabil were collected during 2013 to 2014. The placenta was tested by nested PCR, using fragments of the GRA6 gene. Using PCR method, 17 out of 200(8.5%) samples of spontaneous aborted placenta were found to be positive for T. Gondii. A significant relationship was not observed between the presences of Toxoplasma, mother'sage, and history of abortion, gestational age and illnesses such as allergy, diabetes,hypertension and hypothyroidism.The present findings reveals that Toxoplasma gondii may be one of the potential agents in causing significant rate of abortion in the Ardabil area and placenta analysis is important toimprove the sensitivity of the diagnosis.

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