افزایش بیان Nrf-2 در سلولهای بنیادی مزانشیمی، سیتوتوکسی سیته و آپوپتوز ناشی از استرس اکسیداتیو را کاهش می دهد.

محمدزاده وردین, محمد and حلبیان, راحله and قره باغیان, احمد and امیری زاده, ناصر and جهانیان نجف آبادی, علی and محمدی روشنده, آمنه and حبیبی رودکنار, مهریار (1390) افزایش بیان Nrf-2 در سلولهای بنیادی مزانشیمی، سیتوتوکسی سیته و آپوپتوز ناشی از استرس اکسیداتیو را کاهش می دهد. Cell Stress and Chaperones ــ 17 (5). pp. 553-565. شاپا 8145-1355


Official URL: http://link.springer.com/article/10.1007%2Fs12192-...


Nrf-2 overexpression in mesenchymal stem cells reduces oxidative stress-induced apoptosis and cytotoxicity

English Abstract

The most prominent capabilities of mesenchymal stem cells (MCSs) which make them promising for therapeutic applications are their capacity to endure and implant in the target tissue. However, the therapeutic applications of these cells are limited due to their early death within the first few days following transplantation. Therefore, to improve cell therapy efficacy, it is necessary to manipulate MSCs to resist severe stresses imposed by microenvironment. In this study, we manipulated MSCs to express a cytoprotective factor, nuclear factor erythroid-2 related factor 2 (Nrf2) to address this issue. Full-length human Nrf2 cDNA was isolated and TOPO cloned into TOPO cloning vector and then transferred to gateway adapted adenovirus expression vector by LR recombination reaction. Afterwards, the Nrf2 bearing recombinant virus was prepared in appropriate mammalian cell line and used to infect MSCs. The viability and apoptosis of the Nrf2 expressing MSCs were evaluated following hypoxic and oxidative stress conditions. Transient expression of Nrf2 by MSCs protected them against cell death and the apoptosis triggered by hypoxic and oxidative stress conditions. Nrf2 also enhanced the activity of SOD and HO-1. These findings could be used as a strategy for prevention of graft cell death in MSC-based cell therapy. It also indicates that management of cellular stress responses can be used for practical applications.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محمد محمدزاده وردین
نویسنده :راحله حلبیان
نویسنده :احمد قره باغیان
Additional Information:Impact Factor (2012) 2.484 Indexed in: ISI, Scopus, PubMed/Medline, Embase
کلیدواژه ها (انگلیسی):Mesenchymal stem cells , Nrf2 , Adenoviral vector , Oxidative stress , Apoptosis
Subjects:QS Human Anatomy
WH Hemic and Lymphatic System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:7072
Deposited By: Dr Mohammad Mohammadzadeh
Deposited On:03 Nov 1394 09:12
Last Modified:18 Sep 1399 12:18

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