القاي عصبي سلولهاي بنيادي جنيني موش به دنبال هم كشتي با سومايتهاي جنين جوجه

سقا, محسن and کربلایی, خدیجه and تنهايي, سميه and اسفندياري, ابراهيم and رضوي, شهناز and نصر اصفهاني, محمد حسين and بهاروند, حسين (1388) القاي عصبي سلولهاي بنيادي جنيني موش به دنبال هم كشتي با سومايتهاي جنين جوجه. Stem Cells and Development ــ 18 (9). pp. 1351-1360. شاپا 1547-3287

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Official URL: http://www.liebertonline.com/toc/scd/18/9


Neural Induction in Mouse Embryonic Stem Cells by Co-culturing With Chicken Somites

English Abstract

The role of paraxial mesoderm or the somites in decision of ectoderm to acquire the neuroepithelial fate and its subsequent diversifi cation to functional neural subtypes especially in mammalians is obscure. Here we report, for the fi rst time, the infl uence of the co-culture of alginate bead-encapsulated somites isolated from chick embryos on neural differentiation in mouse embryonic stem cells-derived embryoid bodies (EBs). Using a combination of morphology, immunofl uorescence, fl ow cytometry, semiquantitative, and conventional RT-PCR techniques, we show that the somites induce rosette structures and weakly enhance neural differentiation and neural markers in a dose-dependent manner in comparison to the control group, but its effect is lower than retinoic acid treatment of EBs. By liquid chromatography–mass spectrometry, it was revealed that somitic cells synthesized and released retinoids into the medium. However, no effective infl uence of somitic co-culture on rostrocaudal or dorsoventral patterning is seen. Therefore, like amphibians, somites have a modest neural-inducing activity in amniotes

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محسن سقا
نویسنده مسئول :محمد حسين نصر اصفهاني
Additional Information:Impact Factor=4.146 Indexed/Abstracted in:1-MEDLINE;pubmed-ISI 2-Current Contents®/Life Sciences; Current Contents®/Clinical Medicine; EMBASE/Excerpta Medica; Scopus 3-Biotechnology Citation Index®; EMBiology
کلیدواژه ها (انگلیسی):Neural induction - chicken somites - mouse embryonic stem cells
Subjects:WL Nervous system
QS Human Anatomy
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:716
Deposited By: Dr Mohsen Sagha
Deposited On:25 Oct 1388 06:40
Last Modified:15 Nov 1391 13:20

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