title

اسید رتینوئیک عمل سومایت را در تمایز عصبی ناحیه دمی صفحه عصبی در حال تکوین جنین جوجه تقلید می کند

پیرموذن, الناز and متین, مریم and نجف زاده, نوروز and گل محمدی, محمد قاسم and سقا, محسن (1394) اسید رتینوئیک عمل سومایت را در تمایز عصبی ناحیه دمی صفحه عصبی در حال تکوین جنین جوجه تقلید می کند. Neurochemical Journal ــ 9 (4). pp. 260-265. شاپا 7124-1819

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Title

Retinoic Acid Recapitulates the Action of the Somites on Neural Differentiation of the Developing Caudal Neural Plate in Chick Embryo

English Abstract

During vertebrate neurogenesis, the caudal neural plate (CNP)—consisting of a stem zone— remains open. It adopts with the extension of the body to form the developing spinal cord. In the caudal�most part of the stem zone, the cell population has a dual neural/mesenchymal fate, depending on whether they move to the cranial part of the stem zone or gastrulate to form the underlying presomitic mesoderm. Due to FGF signaling in presomitic mesoderm, the cells residing in the stem zone are in a proliferative state. When the cells migrate cranially to the closing part of the neural tube, they differentiate into neurons. Here, they are exposed to retinoic acid (RA), secreted from the somites. The aim of this study was to evaluate whether RA mimics the somite function in neural differentiation of the CNP in culture conditions. The CNP explants from the chick embryos were dissected, cultured and treated with RA. The untreated CNP was considered as the control group. Using immunostaining and RT�PCR analysis, we examined the MAP2 protein expression and the different genes involved in neural differentiation in both groups. Upon expansion, the CNP�derived cells expressed MAP2 protein. The RT�PCR assessment showed that they also expressed Sox2 and Delta1, as well as Brachyury. Subsequently, the RA induced neural differentiation in the CNP cells through the upreg� ulation of Sox2 and NeuroM and the downregulation of Delta1 and Brachyury. The CNP cells showed a neural progenitor/mesenchymal fate by expressing Sox2, Delta1 and Brachyury. The RA downregulated the Brachyury expression and recapitulated the somite neurogenic function through differentiating the CNP cells into neural�like cells expressing NeuroM.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :الناز پیرموذن
نویسنده :مریم متین
نویسنده مسئول :محسن سقا
Additional Information:Impact Factor(2014) 0.303 Indexed in: ISI, Scopus, Chemical Abstracts Service , Google Scholar, Academic OneFile, ChemWeb, Expanded Academic, OCLC, SCImago, Summon by ProQuest
Uncontrolled Keywords:صفحه عصبی دمی، جنین جوجه، تمایز عصبی، اسید رتینوئیک
کلیدواژه ها (انگلیسی):caudal neural plate, chick embryo, neural differentiation, retinoic acid
Subjects:QS Human Anatomy
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:7168
Deposited By: Dr Mohsen Sagha
Deposited On:14 Sep 1394 07:01
Last Modified:14 Sep 1394 07:01

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