تاثیر پارامترهای مختلف چگالی کشت سلولی بر بقای سلولهای بنیادی مزانشیمی مغز استخوان تحت شرایط استرس اکسیداتیو

Title

The Effect of Different Parameters of Cell Seeding Density on the Survival of Bone Marrow Mesenchymal Stem Cells under Oxidative Stress

English Abstract

BACKGROUND Optimization of cell seeding density in in vitro cell culture conditions has much more importance in mesenchymal stem cells (MSCs) studies. It is especially important in the extension of in vitro results to in vivo activity. The aim of this study was to evaluate the effect of cellular density on MSCs survivals against oxidative stresses. METHODS MSCs were isolated from rat bone marrow and cultured in DMEM-LG and 10% FBS medium. The cells were treated with different concentrations of H2O2 for 4 hours, and cells’ survival rate was estimated with MTT assay. Then, MSCs were cultured in the same condition with the diverse density of 1000 to 4000 cells/96-well plate and treated with IC50 dose of H2O2 for 4 hours. The viability of cells was determined by MTT assay. RESULTS IC50 dose for H2O2 was determined 0.5 mM. Furthermore, this study demonstrated that higher densities of MSCs significantly increase their survival rate against H2O2. CONCLUSTION Our study showed that higher cell seeding density can lead to improve MSCs survival against oxidative stress under in vitro condition, and this can provide new clue in the cell therapy activities.

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