ارزیابی آپوپتوز اولیه در بین سه روش پردازش خون بند ناف

محمدزاده وردین, محمد and نیکوگفتار, مهین and امیری زاده, ناصر and قره باغیان, احمد and سقا, محسن and وائلی, شهرام (1394) ارزیابی آپوپتوز اولیه در بین سه روش پردازش خون بند ناف. در: The 3rd International Congress of Transfusion Medicine, Dec 15-17, 2015, Tehran-Iran.


Official URL: http://www.tmi.ac.ir


Evaluation of Early Apoptosis in Three Cord Blood Processing Methods

English Abstract

BACKGROUND Importance of the hematopoietic stem cell transplantation has directed numerous studies to be done to increase its worth. There is three conventional cord blood processing methods including hydroxyethyl starch, simple centrifugation and Sepax automation. In this study, early apoptosis induced by these processing methods have been compared together. METHODS 90 umbilical cord blood samples were processed by hydroxyethyl starch, centrifugation and Sepax automation methods, and were compared through Syto16/7. AAD assay of apoptosis in CD34+ cells. RESULTS After processing with three methods, significantly more Syto16+/CD34+ cells indicating live cells were yielded with Sepax automation method compared with the two other methods. Also, processing with Sepax automation method had been associated with significantly less early apoptotic (Syto16-/7.AAD-) and late apoptotic (Syto16-/7.AAD+) CD34+ cells. CONCLUSTION Among three umbilical cord blood processing methods, Sepax automation method has the less apoptotic side effects on CD34+ cells.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده مسئول :محمد محمدزاده وردین
نویسنده :محسن سقا
کلیدواژه ها (انگلیسی):Apoptosis, Hematopoietic Stem Cells, Umbilical Cord Blood, Hydroxyethyl Starch Derivatives
Subjects:WH Hemic and Lymphatic System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:7237
Deposited By: Dr Mohammad Mohammadzadeh
Deposited On:10 Oct 1394 07:15
Last Modified:10 Oct 1394 07:15

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