ارزیابی آپوپتوز اولیه در بین سه روش پردازش خون بند ناف

Title

Evaluation of Early Apoptosis in Three Cord Blood Processing Methods

English Abstract

BACKGROUND Importance of the hematopoietic stem cell transplantation has directed numerous studies to be done to increase its worth. There is three conventional cord blood processing methods including hydroxyethyl starch, simple centrifugation and Sepax automation. In this study, early apoptosis induced by these processing methods have been compared together. METHODS 90 umbilical cord blood samples were processed by hydroxyethyl starch, centrifugation and Sepax automation methods, and were compared through Syto16/7. AAD assay of apoptosis in CD34+ cells. RESULTS After processing with three methods, significantly more Syto16+/CD34+ cells indicating live cells were yielded with Sepax automation method compared with the two other methods. Also, processing with Sepax automation method had been associated with significantly less early apoptotic (Syto16-/7.AAD-) and late apoptotic (Syto16-/7.AAD+) CD34+ cells. CONCLUSTION Among three umbilical cord blood processing methods, Sepax automation method has the less apoptotic side effects on CD34+ cells.

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