ارزیابی اثرات محافظت سلولی گیاه مرزنجوش بر بقای سلولهای بنیادی مزانشیمی

محمدزاده وردین, محمد and خوش لحنی, نسرین and پناهپور, حمداله and گل محمدی, محمد قاسم and نجف زاده, نوروز and سقا, محسن (1394) ارزیابی اثرات محافظت سلولی گیاه مرزنجوش بر بقای سلولهای بنیادی مزانشیمی. در: The 3rd International Congress of Transfusion Medicine, Dec 15-17, 2015, Tehran - Iran.


Official URL: http://ibtc.tmi.ac.ir


Evaluation of Cytoprotective Effects of Origanum Vulgare on Mesenchymal Stem Cells Survival

English Abstract

BACKGROUND One of the major challenges in mesenchymal stem cells (MSCs) therapeutic application is their dramatic death following transplantation. In this study, we applied Origanum Vulgare extract as a potent antioxidant to improve MSCs survival under stressful conditions. METHODS MSCs were isolated from rat bone marrow and cultured with DMEM-LG medium and 10% FBS. Following their characterization, the half maximal inhibitory concentration (IC50) of hydrogen peroxide and also maximum safe dose of Origanum Vulgare extract on MSCs were determined by MTT assay. Then, MSCs that had been pretreated with Origanum Vulgare for 48 hours were incubated with IC50 dose of hydrogen peroxide for 4 hour. Following 2 days of recovery time, MTT assay have been done. RESULTS IC50 dose of hydrogen peroxide was 0.55 mM, and also maximum safe dose of Origanum Vulgare was 25 μg/ml. In the combined study, pretreatment with Origanum Vulgare significantly increased MSCs survival. CONCLUSTION This study showed that the culture of MSCs in the presence of Origanum Vulgare extract could improve MSCs survival against oxidative stress and may lead to improve the efficacy of cell therapy

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :محمد محمدزاده وردین
نویسنده :حمداله پناهپور
نویسنده :محمد قاسم گل محمدی
نویسنده :نوروز نجف زاده
نویسنده مسئول :محسن سقا
Uncontrolled Keywords:ماده محافظت کننده سلولی، عصاره گیاهی، گیاه مرزنجوش، سلولهای استرومایی مزانشیمی
کلیدواژه ها (انگلیسی):Cytoprotective Agent, Herbal Extract, Origanum Vulgare, Mesenchymal Stromal Cells
Subjects:QS Human Anatomy
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:7259
Deposited By: Dr Mohsen Sagha
Deposited On:05 Oct 1394 11:01
Last Modified:20 May 1395 07:43

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