مقايسه اثر بخشی دو روش فوتودینامیک تراپی ضد میکروبی بر روی قارچ کاندیدا آلبیکانس درمحیط آزمایشگاهی

فکرآزاد, رضا and قاسمی برقی, ودود and پورستار, آرش and شمس قهفرخی, معصومه (1393) مقايسه اثر بخشی دو روش فوتودینامیک تراپی ضد میکروبی بر روی قارچ کاندیدا آلبیکانس درمحیط آزمایشگاهی. Photodiagnosis and Photodynamic Therapy ــ 12 (1). pp. 52-57. شاپا 1000-1572


Official URL: http://www.sciencedirect.com/science/journal/15721...


In vitro photodynamic inactivation of Candida albicans by phenothiazine dye (New methylene blue) and Indocyanine green (EmunDo®)

English Abstract

Background: The application of a new generation of photosensitizers to increase the efficacyof antifungal photodynamic therapy (aPDT) is an important aspect of PDT. Thus, this in vitrostudy is aimed to evaluate the antifungal efficacy of the photo-elimination of Candida albicanswith photothermal and antifungal photodynamic therapy.Method and material: aPDT with new methylene blue and photothermal therapy with EmunDo®were applied to a fungal suspension, which was then subcultured in Sabouraud dextrose agar(SDA). The C. albicans colonies were counted and are expressed as colony-forming unit permilliliter (CFU/ml).Results: aPDT with either EmunDo®or new methylene blue (NMB) considerably diminished theviability of inoculated C. albicans (P < 0.001) by log reduction of 1.9 and 3.37, respectively,compared with the control group respectively, compared with the control group. The antifungalpotency or dark toxicity of the two photosensitizers alone did not significantly differ (P = 0.70).The same trend was observed for the light sources (�: 810 nm vs. �: 630 nm), which also did notsignificantly differ (P = 0.78).Conclusion: The photo-elimination of C. albicans with either new methylene blue or EmunDo®as a photosensitizer can reduce the viability of fungal cells. Although the result of this study isencouraging, further investigations are warranted to determine clear protocols for the reliableand safe application of this method in clinical practice.© 2015 Elsevier B.V. All rights reserved.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :رضا فکرآزاد
نویسنده مسئول :ودود قاسمی برقی
نویسنده :آرش پورستار
نویسنده :معصومه شمس قهفرخی
Additional Information:Impact Factor(2014) 2.014 Indexed in: ISI, Pubmed/Medline/Index medicus, Scopus, Current Contents / Clinical Medicine, *Original Research Article
کلیدواژه ها (انگلیسی):Antifungal photodynamic therapy; Photothermal therapy, Candida albicans
Subjects:QV pharmacology
Divisions:Department of Dentistry > Department of Oral Surgery, and Maxillofacial
ID Code:7290
Deposited By: Dr Vadoud Ghasemi barghi
Deposited On:13 Feb 1395 11:48
Last Modified:13 Feb 1395 11:48

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