title

سمیت متیل ترشیری بوتیل اتر (MTB) بر روی لنفوسیت های خون انسانی

سلیمی, احمد and وقار موسوی, مهرداد and صیدی, عنایت اله and پوراحمد, جلال (1395) سمیت متیل ترشیری بوتیل اتر (MTB) بر روی لنفوسیت های خون انسانی. Environmental Science and Pollution Research ــ 23 (9). pp. 8556-8564. شاپا 1344-0944

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Official URL: http://link.springer.com/journal/11356


Title

Toxicity of methyl tertiary-butyl ether on human blood lymphocytes

English Abstract

Methyl tertiary-butyl ether (MTBE) is a synthetic solvent widely used as oxygenate in unleaded gasoline. Few studies have addressed the cellular toxicity of MTBE on some cell lines, and so far, no comprehensive study has been conducted to investigate the probable immunotoxicity of this compound. In this study, the toxicity of MTBE on human blood lymphocytes was evaluated. Blood lymphocytes were isolated from healthy male volunteer’s blood, using Ficoll polysaccharide followed by gradient centrifugation. Cell viability, reactive oxygen species (ROS) formation, lipid peroxidation, glutathione levels, and damage to mitochondria and lysosome were determined in blood lymphocytes after 6-h incubation with different concentrations of MTBE (0.1, 0.5, 1, and 2 mM). Our results showed that MTBE, in particular, decreased cell viability, which was associated with significant increase at intracellular ROS level and toxic alterations in mitochondria and lysosomes in human blood lymphocytes. Moreover, it was shown that MTBE strongly provoked lipid peroxidation and also depleted glutathione level at higher concentrations. Interestingly, MTBE exhibited its cytotoxic effects at low concentrations that may resemble to its concentrations in human blood following occupational and environ-mental exposure. It is therefore concluded that MTBE was capable of inducing oxidative stress and damage to mitochondria and lysosomes in human lymphocytes at concentrations ranging from 5 to 40 μg/L, which may be present in human blood as a result of environmental exposure.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :احمد سلیمی
نویسنده :مهرداد وقار موسوی
نویسنده :عنایت اله صیدی
نویسنده مسئول :جلال پوراحمد
Additional Information:IF: 2.741 Indexed in: ISI, PubMed/Medline, Scopus
کلیدواژه ها (انگلیسی):Methyl tertiary-butyl ether, Mitochondria, Oxidative stress, Cytotoxicity, Glutathione, Lysosomes
Subjects:QV pharmacology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:7390
Deposited By: Dr Ahmad Salimi
Deposited On:15 Jan 1395 07:53
Last Modified:23 Jan 1401 11:11

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