title

سمیت انتخابی عصاره متانولی خیار دریایی (Holothuria parva) و اسفنج (Haliclona oculata) خلیج فارس بر روی میتوکندری های ایزوله شده ازیک مدل حیوانی مبتلاء به هپاتوسولار کارسینوما

صیدی, عنایت اله and مطلبی, عباس علی and دست باز, مریم and دهقان, سحر and سلیمی, احمد and ناظمی, ملیکا and پوراحمد, جلال (1394) سمیت انتخابی عصاره متانولی خیار دریایی (Holothuria parva) و اسفنج (Haliclona oculata) خلیج فارس بر روی میتوکندری های ایزوله شده ازیک مدل حیوانی مبتلاء به هپاتوسولار کارسینوما. Hepatitis Monthly ــ 15 (12). pp. 1-12. شاپا 143x-1735

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Title

Selective Toxicity of Persian Gulf Sea Cucumber (Holothuria parva) and Sponge (Haliclona oculata) Methanolic Extracts on Liver Mitochondria Isolated from an Animal Model of Hepatocellular Carcinoma

English Abstract

Background: Natural products isolated from marine environments are well known for their pharmacodynamic potential in diverse disease treatments, such as for cancer or inflammatory conditions. Sea cucumbers are marine animals of the phylum Echinoderm and the class Holothuroidea, with leathery skin and gelatinous bodies. Sponges are important components of Persian Gulf animal communities, and the marine sponges of the genus Haliclona have been known to display broad-spectrum biological activity. Many studies have shown that sea cucumbers and sponges contain antioxidants and anti-cancer compounds. Objectives: This study was designed to determine the selective toxicity of Persian Gulf sea cucumber (Holothuria parva) and sponge (Haliclona oculata) methanolic extracts on liver mitochondria isolated from an animal model of hepatocellular carcinoma, as part of a national project that hopes to identify novel potential anticancer candidates among Iranian Persian Gulf flora and fauna. Materials and Methods: To induce hepatocarcinogenesis, rats were given diethylnitrosamine (DEN) injections (200 mg/kg i.p. by a single dose), and then the cancer was promoted with 2-acetylaminofluorene (2-AAF) (0.02 w/w) for two weeks. Histopathological evaluations were performed, and levels of liver injury markers and a specific liver cancer marker (alpha-fetoprotein), were determined for confirmation of hepatocellular carcinoma induction. Finally, mitochondria were isolated from cancerous and non-cancerous hepatocytes. Results: Our results showed that H. parva methanolic extracts (250, 500, and 1000 µg/mL) and H. oculata methanolic extracts (200, 400, and 800 µg/mL) increased reactive oxygen species (ROS) formation, mitochondrial membrane potential (MMP), mitochondrial swelling, and cytochrome c release in the mitochondria obtained from cancerous hepatocytes, but not in mitochondria obtained from non-cancerous liver hepatocytes. These extracts also induced caspase-3 activation, which is known as a final mediator of apoptosis, in the hepatocytes obtained only from cancerous, not non-cancerous, rat livers. Conclusions: Our results suggest that H. parva and H. oculata may be promising therapeutic candidates for the treatment of HCC, following further confirmatory in vivo experiments and clinical trials.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :عنایت اله صیدی
نویسنده :احمد سلیمی
نویسنده مسئول :جلال پوراحمد
Additional Information:Impact Factor (2014) 1.932 Indexed in: ISI, Pubmed, Scopus, DOAJ, Index Copernicus, World Cat, EBSCO HOST, CINAHL, Open-J-Gate, Google Scholar, Embase, Iran Medex, MagIran, SID, ISC, Research Article
کلیدواژه ها (انگلیسی):Carcinoma, Hepatocellular, Hepatocytes, Mitochondria, Holothuria parva, Haliclona oculata
Subjects:QV pharmacology > QV 600 Toxicology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:7461
Deposited By: دکتر احمد سلیمی
Deposited On:15 Dec 1394 10:44
Last Modified:15 Dec 1394 10:44

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