مهار اگزوتوکسین تبزای Bاسترپتوکوکوس پیوژنز توسط آلیسین از سیر

ارزنلو, محسن (1394) مهار اگزوتوکسین تبزای Bاسترپتوکوکوس پیوژنز توسط آلیسین از سیر. Microbial Pathogenesis ــ 93 . pp. 166-171. شاپا 0882-4010

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Official URL: http://authors.elsevier.com/a/1ScSj38ed-4GHS


Inhibition of streptococcal pyrogenic exotoxin B using allicin from garlic

English Abstract

Streptococcal pyrogenic exotoxin B (SpeB) is an important virulence factor of group A streptococci (GAS) and inactivation of SpeB results in the significantly decreased virulence of the bacterium. The protein is secreted as an inactive zymogen of 40 KDa (SpeBz) and undergoes proteolytic truncation to result in a 28 KDa mature active protease (SpeBm). In this study the effect of allicin on the proteolytic activity of SpeBm was evaluated using azocasein assay. Allicin neutralized the SpeBm proteolytic activity in a concentration dependent manner (IC50 ¼ 15.71 ± 0.45 mg/ml). The loss of activity was completely reversed by subsequent treatment with a reducing agent, dithiothreitol (DTT; 10 mM final concentration), suggesting that allicin likely inhibits the SpeBm by forming a disulfide linkage with an active thiol group in its active site. This mechanism of action was further confirmed with the fact that DTT did not reverse the SpeBm activity in the presence of E�64, a cysteine protease-specific inhibitor, which works specially by forming a thioether linkage with free sulfhydryl groups in enzymes active site. The MIC of allicin against GAS was found to be 32 mg/ml. Exposure of GAS culture to allicin (25 mg/ml) inhibited maturation of SpeBz to the SpeBm. In conclusion, the results of this study suggest that allicin inhibits the maturation of SpeBz and proteolytic activity of SpeBm and could be a potential therapeutic agent for the treatment of GAS infections.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محسن ارزنلو
Additional Information:Impact Factor (2014) 1.794 Indexed in: ISI, Pubmed/ , Medline/Index Medicus, Scopus, Chemical Abstracts , BIOSIS, Current Contents/Life Sciences, Microbiology Abstracts, Genetics Abstracts, Immunology Abstracts, Biological Abstracts, Original Research Article
کلیدواژه ها (انگلیسی):Group A streptococci,SpeB,Zymogen,Garlic,Allicin
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:7462
Deposited By: Dr Mohsen Arzanlou
Deposited On:15 Dec 1394 07:18
Last Modified:15 Dec 1394 10:23

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