title

پیوند سلولهای بنیادی مزانشیمی افزایش بیان یافته ژن Nrf2، در موشهای صحرایی دچار آسیب حاد کلیوی ناشی از گلیسرول، عملکرد کلیوی را بهبود می بخشد و شاخصهای آسیب را کاهش و شاخصهای ترمیم را افزایش می دهد.(2016)

ژاله, فاطمه and امیری, فاطمه and محمدزاده وردین, محمد and بهادری, مرضیه and دهقان هراتی, میترا and حبیبی رودکنار, مهریار and ساکی, ساسان (1395) پیوند سلولهای بنیادی مزانشیمی افزایش بیان یافته ژن Nrf2، در موشهای صحرایی دچار آسیب حاد کلیوی ناشی از گلیسرول، عملکرد کلیوی را بهبود می بخشد و شاخصهای آسیب را کاهش و شاخصهای ترمیم را افزایش می دهد.(2016). Iranian Journal of Basic Medical Sciences ــ 19 (3). pp. 323-329. شاپا 3866-2008

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Title

Nuclear factor erythroid-2 related factor 2 overexpressed mesenchymal stem cells transplantation, improves renal function, decreases injuries markers and increases repair markers in glycerol-induced Acute kidney injury rats

English Abstract

Objective(s):Recently cell therapy is a promising therapeutic modality for many types of disease including acute kidney injury (AKI). Due to the unique biological properties, mesenchymal stem cells (MSCs) are attractive cells in this regard. This study aims to transplant MSCs equipped with nuclear factor E2-related factor 2 (Nrf2) in rat experimental models of acute kidney and evaluate regeneration potential of injured kidney especially expression of injury and repaired biomarkers. Materials and methods:Nrf2 was overexpressed in bone marrow-derived MSCs by pcDNA.3.1 plasmid. AKI was induced using glycerol in rat models. The regenerative potential of Nrf2-overexpressed MSCs was evaluated in AKI-Induced animal models using biochemical and histological methods after transplantation. Expression of repaired genes, AQP1 and CK-18, as well as injury markers, Kim-1 and Cystatin C, was also assayed in engrafted kidney sections. Results:Our results revealed that transplantation of Nrf2-overexpressed MSCs into AKI-induced rats decreased blood urea nitrogen and creatinine and ameliorated kidney regeneration throughout 14 days. Upregulation of repaired markers and downregulation of injury markers were considerable 14 days after transplantation. Conclusions: Overexpression of Nrf2 in MSCs suggests a new strategy to increase efficiency of MSC-based cell therapy in AKI.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :فاطمه ژاله
نویسنده :محمد محمدزاده وردین
نویسنده مسئول :ساسان ساکی
Additional Information:Impact Factor (2014) 1.288 Indexed in: ISI, Pubmed, Scopus, BIOSIS Previews, EMBASE, Chemical Abstracts, ISC, *Original article
کلیدواژه ها (انگلیسی):Acute kidney injury; AQP1; Kim-1; Mesenchymal stem cells; Nrf2
Subjects:QS Human Anatomy
QZ Pathology
WJ Urogenital System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:7520
Deposited By: Dr Mohammad Mohammadzadeh
Deposited On:19 Jan 1395 05:12
Last Modified:19 Jan 1395 05:12

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