title

آزلائيك اسيداز طریق کاهش رشد فیبروبلاست می تواند به رژنراسیون اعصاب محیطی کمک کند

شریفی پاسندی, مرضیه and محمدی, پرهام سعداله and نیاپور, علی (1395) آزلائيك اسيداز طریق کاهش رشد فیبروبلاست می تواند به رژنراسیون اعصاب محیطی کمک کند. در: The 5th Symposium of World Federation of Neurosurgical Societies, Apr 17-22, 2016, Tehran- Iran.

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Title

Azelaic acid could promote peripheral nerve regeneration through reducing fibroblast growth

English Abstract

Background and Aim: Traumatic injury to the peripheral nerve results in neural dysfunctions and neuropathy. A series of cellular and molecular events takes place before regeneration of injured peripheral nerves. During Wallerian degeneration, Schwann cellderived TNFα and IL-1α induce resident fibroblasts to further produce and liberate cytokines such as IL-6 and GM-CSF following 2 to 5 hours after the injury. Meanwhile, fibroblasts proliferate and accumulate at the injury site and may participate in neuroma tissue formation. Azelaic acid (AzA) which is being used for the treatment of inflammatory skin diseases like acne and rosacea has inhibitory effects on pro-inflammatory cytokines such as IL-1, IL-6 and TNF-α release. Therefore, the aim of this study was to evaluate the possible effects of the AzA on rat peripheral nerve fibroblasts viability. Methods: Rat peripheral nerve fibroblasts were seeded at a density of 1000 cells per well in 96 well plates. After 24 hours, cells were incubated with different concentrations of the AzA (from 0.1μM to 10 mM) for 48 hours. Cellular viability was determined using MTT assay. Results: Our findings demonstrated that after 48 hours of treatment with 2.5 and 5mM of AzA, significant reduction of cellular viability were observed. However, concentrations lower than 2.5mM showed minimal impacts on reducing rat peripheral nerve fibroblasts growth. The 5th Symposium of World Federation of 422 Neurosurgical Societies Conclusion: AzA had a cytotoxic effect in rat peripheral nerve fibroblasts. Our data provides an in vitro evidence of azelaic acid in prevention of neuroma tissue formation.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :مرضیه شریفی پاسندی
نویسنده :پرهام سعداله محمدی
نویسنده مسئول :علی نیاپور
کلیدواژه ها (انگلیسی):Azelaic acid, rat peripheral nerve fibroblasts, MTT.
Subjects:QS Human Anatomy
QV pharmacology
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
Faculty of Medicine > Department of Basic Sciences > Department of Pharmacology
School of Pharmacy > Department of Pharmacology
ID Code:7614
Deposited By: Dr Ali Niapour
Deposited On:29 Feb 1395 06:05
Last Modified:12 Dec 1397 13:52

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