تمایز بهتر سلولهای بنیادی پرتوان CD34 مثبت فولیکول مو با استفاده از رتینوئیک اسید و محیط بدون سرم

سقا, محسن and نجف زاده, نوروز (1395) تمایز بهتر سلولهای بنیادی پرتوان CD34 مثبت فولیکول مو با استفاده از رتینوئیک اسید و محیط بدون سرم. در: Multipotent Stem Cells of the Hair Follicle. Methods and Protocols . Humana press, New York, pp. 172-161. شابک 4-3784-4939-1-978

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Official URL: http://www.springer.com/us/book/9781493937844


Highly Efficient Neural Differentiation of CD34-Positive Hair-Follicle-Associated Pluripotent Stem Cells Induced by Retinoic Acid and Serum-Free Medium

English Abstract

Neural differentiation of hair-follicle-associated pluripotent (HAP) stem cells residing in the bulge area is a promising autologous source for stem cell therapy. In the present chapter, we describe the identification and enrichment of CD34 + HAP stem cells by magnetic-activated cell sorting (MACS), and induce them to differentiate into neuronal and glial cells using defined neural-induction media. The different neural cell populations arising during in vitro differentiation from HAP stem cells are characterized by reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry assay.

Item Type:Book Section
زبان سند : انگلیسی
نویسنده اول :محسن سقا
نویسنده مسئول :نوروز نجف زاده
کلیدواژه ها (انگلیسی):Hair follicle,Stem cells, Neural differentiation, Magnetic-activated cell sorting, CD34 + hair-follicle-associated-pluripotent (HAP) stem cell
Subjects:QS Human Anatomy
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:7708
Deposited By: Dr Nowruz Najafzadeh
Deposited On:20 May 1395 07:25
Last Modified:11 Aug 1399 08:48

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