سمیت انتخابی اپیجنین بر روی هپاتوسیت های سرطانی با هدف قرار دادن مستقیم میتوکندری های آنها

صیدی, عنایت اله and راسخ, حمید رضا and سلیمی, احمد and محسنی فر, ژاله and پوراحمد, جلال (1395) سمیت انتخابی اپیجنین بر روی هپاتوسیت های سرطانی با هدف قرار دادن مستقیم میتوکندری های آنها. Anti-Cancer Agents in Medicinal Chemistry ــ 16 (12). pp. 1576-1586. شاپا 1871-5206


Official URL: http://www.eurekaselect.com/article/75152


Selective Toxicity of Apigenin on Cancerous Hepatocytes by Directly Targeting their Mitochondria

English Abstract

Background: hepatocellular carcinoma (HCC) is the third cause of mortality due to cancer throughout the world. Objective: The main goal of the current research was to evaluate the selective toxicity of apigenin (APG) on hepatocytes and mitochondria obtained from the liver of HCC rats. Method: In this research, HCC induced by a single dose of diethylnitrosamine (DEN); 200 mg/kg, i.p, and 2-acetylaminofluorene (2-AAF) (0.02%, through dietary) for 14 days. For confirmation of HCC, histopathological evaluations and determination of serum concentrations of liver toxicity enzymes and specific liver cancer marker; alpha-fetoprotein (AFP) were performed. Then, cancerous and non- cancerous hepatocytes were isolated by using the collagen perfusion method. Eventually, mitochondria isolated from HCC and normal hepatocytes were tested for every eventual toxic effects of APG. Results: After confirmation of HCC, the results of this research showed that APG (10, 20 and 40 μM) increased mitochondrial parameters such as, mitochondrial membrane potential (MMP), reactive oxygen species (ROS) level,mitochondrial swelling and cytochrome c expulsion only in cancerous hepatocytes. Apoptotic effect of APG on HCC cells was confirmed by caspase-3 activation and Annexin V-FITC and PI double staining analysis. Conclusion: These results propose the eligibility of the flavonoid APG as a complementary therapeutic agent for patients with hepatocellular carcinoma.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :عنایت اله صیدی
نویسنده :حمید رضا راسخ
نویسنده :احمد سلیمی
نویسنده :ژاله محسنی فر
نویسنده مسئول :جلال پوراحمد
Additional Information:IF: 2.598 Indexed in: ISI, PubMed/Medline, Scopus, Embase
کلیدواژه ها (انگلیسی):Apigenin, apoptosis, hepatocytes, mitochondria, hepatocellular carcinoma
Subjects:QV pharmacology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:7709
Deposited By: Dr Ahmad Salimi
Deposited On:14 Sep 1395 08:41
Last Modified:25 Jan 1401 09:01

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