بررسی هیپرمتیلاسیون ژن p16 در سرم بیماران مبتلا به سرطان معده در استان اردبیل

وثوقی, ابراهیم and اصولی, رویا and موسوی, زهرا and مأذنی, محمد and اخوان, هما and الیاسی, فریماه and صدوق, سها and اصغراوا, طاهیرا and حسینی اصل, سید سعید (1395) بررسی هیپرمتیلاسیون ژن p16 در سرم بیماران مبتلا به سرطان معده در استان اردبیل. International Journal of Medical Research & Health Sciences ــ 5 (7S). pp. 30-33. شاپا 5886-2319


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Investigation of P16 gene hypermethylation in serum of gastric cancer patients in Ardabil province

English Abstract

Gastric cancer is the second leading cause of cancer death in the world. There is evidence that shows genetic and epigenetic change in stomach cancer is as a result of a variety of oncogenes, tumor suppressor genes, genes that repair the DNA, genes regulating cell cycle and cell adhesion molecules. P16 is an Inhibitor of protein kinase that is dependent on Cyclin D. Inactivation of this gene as a tumor suppressor gene, cause to cancer. This study was conducted to Investigation the P16 hyper-metylation status in serum of gastric cancer patients in Ardabil province as the prognostic factor for early diagnosis of gastric cancer. 82 Blood samples of patients with gastric cancer was prepared from Aras clinic of Imam Khomeini hospital. After DNA extraction from serum, using methylation-specific PCR assay it was examined the existence of hypermethylation in the P16 gene promoter. Incidence of free DNA in the serum of patients was 59.8%. P16 gene promoter was hyper methylated in 20.4% of subjects. Data analysis showed a statistically significant association between P16 methylation status and types of the adenocarcinoma. But there was no statistically significant association between methylation status of P16 with other features (age, sex, tumor location). This study showed P16 gene methylation of occur in some of gastric cancer patients. This result suggests that p16 methylation may be a prognostic marker for early diagnosis of gastric cancer.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :ابراهیم وثوقی
نویسنده :محمد مأذنی
نویسنده مسئول :سید سعید حسینی اصل
Additional Information:Indexd in: Index Copernicus, scopemed,Google Scholar, . Open J Gate, DOAJ,
کلیدواژه ها (انگلیسی):gastric cancer, P16 gene hypermethylation, MS-PCR
Subjects:QY Clinical Pathology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Genetics
ID Code:7812
Deposited By: Dr S Saied Hosseini-Asl
Deposited On:18 Jun 1395 07:46
Last Modified:18 Jun 1395 07:46

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