با استفاده از پیش شرطی سازی گیاهی، عصاره گیاه مرزنجوش، سلولهای بنیادی مزانشیمی را در برابر استرس اکسیداتیو محافظت می کند.

Title

Using Herbal Preconditioning, Extract of Origanum Vulgare Protects the Mesenchymal Stem Cells from the Oxidative Stress

English Abstract

BACKGROUND: One of the most important challenges in mesenchymal stem cells (MSCs) mediated cell therapy is their low survival rate following transplantation. Therefore, a great number of preconditioning studies have been conducted to potentiate the MSCs against stresses. This study was aimed at preconditioning of the MSCs by using the high concentrations of Origanum Vulgare extract to protect them against oxidative stress. METHODS: MSCs were isolated from the bone marrow of rat. Then, they were preconditioned with the high doses (80-0.625 mg/ml) of Origanum Vulgare extract for one hour. Following two days recovery period, MSCs were exposed to the half maximal inhibitory concentration (IC50) of hydrogen peroxide as an oxidative stress agent for four hours. After two days recovery again, MSCs survival and apoptosis were evaluated by using MTT and caspase 3 assays, respectively. RESULTS: Preconditioning of the MSCs with Origanum Vulgare extract at2.5mg/ml significantly increased the MSCs survival from 46% in H2O2-induced MSCs to 74% preconditioned-MSCs and significantly decreased their caspase 3 from 229% in H2O2-induced cells to 116% when they were preconditioned with Origanum Vulgare extract (p<0.05) CONCLUSIONS: Herbal preconditioning with the higher doses of Origanum Vulgare can protect mesenchymal stem cells against oxidative stress and this strategy may be suggested to prevent the cell death in transplantation programs.

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