آنالیز مقایسه ای فراوانی و توزیع سلولهای پیش ساز وبنیادی عصبی در مغز موش بالغ

گل محمدی, محمد قاسم and بلکمور, دانیل and لارج, بیترس and آذری, حسن and اسفندياري, ابراهيم and پاکسینوز, جورج and فرانکلین, کیت and رینولدز, برنت and ریتز, رادنی لی (1386) آنالیز مقایسه ای فراوانی و توزیع سلولهای پیش ساز وبنیادی عصبی در مغز موش بالغ. Stem Cells ــ 26 (4). pp. 979-987. شاپا 1066-5099

Text - Published Version

Official URL: http://www3.interscience.wiley.com/journal/1215874...


Comparative Analysis of the Frequency and Distribution of Stem and Progenitor Cells in the Adult Mouse Brain

English Abstract

cells (NSCs) and progenitor cells, but it cannot discriminate between these two populations. Given two assays have purported to overcome this shortfall, we performed a comparative analysis of the distribution and frequency of NSCs and progenitor cells detected in 400 m coronal segments along the ventricular neuraxis of the adult mouse brain using the neurosphere assay, the neural colony forming cell assay (N-CFCA), and label-retaining cell (LRC) approach. We observed a large variation in the number of progenitor/stem cells detected in serial sections along the neuraxis, with the number of neurosphereforming cells detected in individual 400 m sections varying from a minimum of eight to a maximum of 891 depending upon the rostral-caudal coordinate assayed. Moreover, the greatest variability occurred in the rostral portion of the lateral ventricles, thereby explaining the large variation in neurosphere frequency previously reported. Whereas the overall number of neurospheres (3730 276) or colonies (4275 124) we detected along the neuraxis did not differ significantly, LRC numbers were significantly reduced (1186 188, 7 month chase) in comparison to both total colonies and neurospheres. Moreover, approximately two orders of magnitude fewer NSC-derived colonies (50 10) were detected using the N-CFCA as compared to LRCs. Given only 5% of the LRCs are cycling (BrdU/Ki-67) or competent to divide (BrdU/Mcm-2), and proliferate upon transfer to culture, it is unclear whether this technique selectively detects endogenous NSCs. Overall, caution should be taken with the interpretation and employment of all these techniques.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محمد قاسم گل محمدی
نویسنده مسئول :رادنی لی ریتز
Additional Information:Impact Factor: 7.531 Indexed:1- ISI,MEDLINE/PubMed 2-Scopus-Index medicus Online ISSN: 1549-4918
کلیدواژه ها (انگلیسی):Neural stem cell , Precursor , Bromodeoxyuridine , Label-retaining cell , Neurosphere
Subjects:WL Nervous system
QS Human Anatomy
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:782
Deposited By: Dr Mohammad Gasem Golmohammani
Deposited On:29 Oct 1388 09:50
Last Modified:07 Jul 1393 06:26

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