طراحی و ساخت DNAواکسن بر اساس پروتئین های ساختمانی ویروس هپاتیت C

تیمورپور, رقیه and تاجانی, امینه سادات and عسگری, وحید رضا and رستمی, سینا and مشکات, زهرا (1395) طراحی و ساخت DNAواکسن بر اساس پروتئین های ساختمانی ویروس هپاتیت C. Iranian journal of pathology ــ 11 (3). pp. 222-230. شاپا 1735-5303

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Designing and Development of a DNA Vaccine Based On Structural Proteins of Hepatitis C Virus

English Abstract

Background: Hepatitis C virus (HCV) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. Therefore, designing new and effective therapeutics is of great importance. The aim of the current study was to construct a DNA vaccine containing structural proteins of HCV and evaluation of its expression in an eukaryotic system. Methods: Structural proteins of HCV (core, E1, and E2) were isolated and amplified from JFH strain of HCV genotype 2a using PCR method. The PCR product was cloned into pCDNA3.1(+) vector and finally were confirmed by restriction enzyme analysis and sequencing methods. The eukaryotic expression of the vector was confirmed by RT-PCR. Results: A recombinant vector containing 2241bp fragment of HCV structural genes was constructed. The desired plasmid was sequenced and corresponded to 100% identity with the submitted sequences in GenBank. RT-PCR results indicated that the recombinant plasmid could be expressed efficiently in the eukaryotic expression system. Conclusion: Successful cloning of structural viral genes in pCDNA3.1(+) vector and their expression in an eukaryotic expression system facilitates the development of new DNA vaccines against HCV. A DNA vaccine encoding core-E1-E2 antigens was designed. The desired expression vector can be used for further attempts in the development of vaccines.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :رقیه تیمورپور
نویسنده مسئول :زهرا مشکات
Additional Information:Indexed in: Pubmed/PMC, Scopus, ISC, DOAJ, SID, EBSCO, magiran, IranMedex, Google Scholar,
کلیدواژه ها (انگلیسی):DNA Vaccine; HCV; Structural proteins
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:7875
Deposited By: Dr Roghayeh Teimourpour
Deposited On:23 Sep 1395 11:14
Last Modified:06 May 1399 09:31

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