مقایسه اثرات کلوفیبرات و آنالوگ جدید سیلیکون دار بر روی هپاتوسیتهای ایزوله موش صحرایی

ضیایی, مجتبی and اقبال, محمد علی and غفار زاده, محمد and عندلیب, سینا and مصطفی لو, سارا and خرمی, آرش (1390) مقایسه اثرات کلوفیبرات و آنالوگ جدید سیلیکون دار بر روی هپاتوسیتهای ایزوله موش صحرایی. در: 20th Iranian Congress of Physiology and Pharmacology, Oct 10-13 , 2011, hamedan - Iran.

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Comparative effects of clofibrate and its novel siliconized analogue in isolated rat hepatocytes

English Abstract

Peroxisome proliferator ligands have been found to have hepatoprotective effect against hepatotoxicants induced injuries. The present investigation aimed to study and compare the effects of clofibrate and its newly synthesized analogue, silafibrate on freshly isolated rat hepatocytes. A siliconized analogue of clofibrate was synthesized, whereby the chlorine atom in the phenoxy ring was replaced by trimethylsily and ethyl2-methyl-2- (4-(trimethylsilyl)phenoxy)propionate was prepared. FeIII:8HQ complex elevated ROS formation in hepatocytes. Application of clofibrate at concentrations of 200 ?M rapidly protected hepatocytes against FeIII:8HQ induced oxidative stress in freshly isolated cells, while silafibrate of 200 ?M had better protective effect. Levels of reactive oxygen species (ROS) were measured with the fluorescent probes 2?,7?- dichlorofluorescein diacetate (DCFHDA). Clofibrate at 200, 400 ?M concentrations reduced reactive oxygen species formation. Treatment of the hepatocytes with silafibrate caused a significant decrease in ROS formation in comparison with clofibrate. ??m happened just before cell death and seems to involve the mitochondrial permeability transition pore (MPTP).Consistent with this, fibrates increased hepatic mitochondrial membrane potential (??m). Mitochondrial membrane potential was measured by using Rhodamine 123 fluorescence. Treatment of the hepatocytes with silafibrate led to significant increase in mitochondrial membrane potential (??m) in comparison with clofibrate. These results suggest that fibrates have hepatoprotective effect against oxidative stress. Silafibrate, the novel analogue, has better effect in protection against oxidative stress in comparison with clofibrate.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :مجتبی ضیایی
نویسنده :سارا مصطفی لو
Uncontrolled Keywords:کلوفیبرات، سیلافیبرات، هپاتوسیت های ایزوله، استرس اکسیداتیو
کلیدواژه ها (انگلیسی):Clofibrate, Silafibrate, Isolated Hepatocytes, Oxidative stress
Subjects:QV pharmacology > QV 600 Toxicology
QV pharmacology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:7883
Deposited By: Dr Sara Mostafalou
Deposited On:16 Aug 1395 12:12
Last Modified:16 Aug 1395 12:12

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