اثرات محافظتی ان-استیل سیستئین بر آسیب میتوکندریائی و گونه های فعال اکسیژن القا شده با مالاتیون در سلولهای کبدی موش صحرائی

اقبال, محمد علی ، مصطفی لو, سارا ، عبدالهی, محمد ، سعیدی کوزه کنانی, نازلی ، ضیائی, مجتبی (1390) اثرات محافظتی ان-استیل سیستئین بر آسیب میتوکندریائی و گونه های فعال اکسیژن القا شده با مالاتیون در سلولهای کبدی موش صحرائی. در: 11th International of the Iranian Society of Toxicology, 2011, Sept 13 -15, Mashad - Iran.

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آدرس اینترنتی رسمی : http://www.mums.ac.ir/toxicology

عنوان انگليسي

The Protective Effect of NAC against "Malathion – Induced" ROS Formation and Mitochondrial Dysfunction in Freshly Isolated Rat Hepatocytes

خلاصه انگلیسی

PURPOSE: Induction of oxidative stress by Organophosphate compounds (OPs) has been previously reported. In the present work, the mechanism of protective effects of N-acetylcysteine as a glutathion (GSH) prodrug against malathion-induced cell toxicity was investigated. In this work, freshly isolated rat hepatocytes were used to determine the effect of NAC on malathion-induced cytotoxicity, formation of reactive oxygen species (ROS) and mitochondrial dysfunction. METHODS: Rat hepatocytes were isolated using collagenase perfusion and then cell viability, mitchondrial membrane potential (MMP) and ROS formation were determined using trypan blue exclusion, Rhodamine 123 fluorescence and fluorogenic probe, 2', 7' -dichlorofluorescin diacetate (DCFH-DA), respectively. RESULTS: Despite the protective effect of NAC on malathion-induced cell toxicity and MMP dysfunction, its efficacy against ROS formation was not adequate to completely protect the cells. CONCLUSION: Cytotoxic effects of malathion regardless of its cholinergic feature, is started with gradual free radical production but, the main factor that causes cell death, is mitochondrial dysfunction, so that reduction of ROS formation alone is not sufficient for cell survival, and the maintenance of mitochondrial integrity through different mechanisms is the most ameliorative factor specially at high levels of cell damage, as NAC seemed to protect cells with various fashions apart from ROS scavenging in concentrations higher than malathion's LC50

نوع سند :موضوع کنفرانس یا کارگاه (سخنرانی )
زبان سند : انگلیسی
نویسنده اول :محمد علی اقبال
نویسنده :سارا مصطفی لو
کلید واژه ها (انگلیسی):Malathion, toxicity, hepatic, mitochondria, reactive oxygen species
موضوعات :QV فارماکولوژی > QV 600 سم شناسی
QV فارماکولوژی
بخش های دانشگاهی :دانشکده داروسازی > بخش سم شناسی
کد شناسایی :7965
ارائه شده توسط : دکتر سارا مصطفی لو
ارائه شده در تاریخ :09 آبان 1395 09:24
آخرین تغییر :02 آذر 1395 11:21

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