اثرات محافظتی ان-استیل سیستئین بر آسیب میتوکندریائی و گونه های فعال اکسیژن القا شده با مالاتیون در سلولهای کبدی موش صحرائی

اقبال, محمد علی and مصطفی لو, سارا and عبدالهی, محمد and سعیدی کوزه کنانی, نازلی and ضیائی, مجتبی (1390) اثرات محافظتی ان-استیل سیستئین بر آسیب میتوکندریائی و گونه های فعال اکسیژن القا شده با مالاتیون در سلولهای کبدی موش صحرائی. در: 11th International of the Iranian Society of Toxicology, 2011, Sept 13 -15, Mashad - Iran.


Official URL: http://www.mums.ac.ir/toxicology


The Protective Effect of NAC against "Malathion – Induced" ROS Formation and Mitochondrial Dysfunction in Freshly Isolated Rat Hepatocytes

English Abstract

PURPOSE: Induction of oxidative stress by Organophosphate compounds (OPs) has been previously reported. In the present work, the mechanism of protective effects of N-acetylcysteine as a glutathion (GSH) prodrug against malathion-induced cell toxicity was investigated. In this work, freshly isolated rat hepatocytes were used to determine the effect of NAC on malathion-induced cytotoxicity, formation of reactive oxygen species (ROS) and mitochondrial dysfunction. METHODS: Rat hepatocytes were isolated using collagenase perfusion and then cell viability, mitchondrial membrane potential (MMP) and ROS formation were determined using trypan blue exclusion, Rhodamine 123 fluorescence and fluorogenic probe, 2', 7' -dichlorofluorescin diacetate (DCFH-DA), respectively. RESULTS: Despite the protective effect of NAC on malathion-induced cell toxicity and MMP dysfunction, its efficacy against ROS formation was not adequate to completely protect the cells. CONCLUSION: Cytotoxic effects of malathion regardless of its cholinergic feature, is started with gradual free radical production but, the main factor that causes cell death, is mitochondrial dysfunction, so that reduction of ROS formation alone is not sufficient for cell survival, and the maintenance of mitochondrial integrity through different mechanisms is the most ameliorative factor specially at high levels of cell damage, as NAC seemed to protect cells with various fashions apart from ROS scavenging in concentrations higher than malathion's LC50

Item Type:Conference or Workshop Item (Speech)
زبان سند : انگلیسی
نویسنده اول :محمد علی اقبال
نویسنده :سارا مصطفی لو
کلیدواژه ها (انگلیسی):Malathion, toxicity, hepatic, mitochondria, reactive oxygen species
Subjects:QV pharmacology > QV 600 Toxicology
QV pharmacology
Divisions:School of Pharmacy > Department of Toxicology
ID Code:7965
Deposited By: Dr Sara Mostafalou
Deposited On:09 Aug 1395 09:24
Last Modified:02 Sep 1395 11:21

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