بررسی نقش آنزیم NADPH-اکسیداز در سمیت دیازینون در موش صحرائی

Title

Role of Nicotinamid Adenine Dinucleotide Phosphate-Oxidase (NADPH-Oxidase) Enzyme Activity in Multi Organ Toxicity of Diazinon in Rat

English Abstract

Objective: Diazinon is one of the most widely used organophosphate insecticides (OPI) for agriculture and public health programs. The aim of this study is to assess the role of NADPH oxidase activity as a key enzyme in oxidative stress processes in liver, pancreas and heart of rat diazinon subacute poisoning. Methods: Mature male Wistar rats were given diazinon (35 and 70 mg/kg; 1/10 and 1/5 of the LD50, respectively) via gavage for 4 weeks. At the end of the treatment, the liver, pancreas and heart tissues were analyzed for Reactive oxygen species (ROS), NADPH oxidase activity and biochemical serum markers, Alanine transaminase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Amylases and Lactate dehydrogenase (LDH) were taken from rats at four weeks. Result: Diazinon (70 mg/kg) in comparison with control group significantly induced NADPH oxidase activity in the heart (p<0.05) and liver (p<0.05) that it positively affected oxidant/antioxidant system (a decline in the levels of total antioxidant capacity and increase of ROS). These effects showed a close relation to increased hepatic enzymes, ALT (p<0.01), AST (p<0.01), ALP (p<0.01), and CPK (p<0.05) as a cardiac marker toxicity. Despite, increasing of ROS (p<0.001) in pancreas tissue and serum amylase (p<0.001), no significant change was observed in NADPH oxidase activity.

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