امانی, مجتبی (1395) همخوانی انطباق داده های DSC با مدل کامل Lumry-Eyring و مدل دو حالته. در: 14th conference on Biophysical Chemistry, 2016 ,Nov 25 - 27, Zabol - Iran.
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Official URL: http://cbc14.uoz.ac.ir/
Title
onsistency of fitting the DSC data to complete Lumry-Eyring and Two-state models
English Abstract
The consistency of the results of fitting the DSC data to complete Lumry-Eyring and Two-state models was assessed for cupper containing amine oxidase from Euphorbia characias (ELAO). Thermal denaturation of ELAO was reported to be irreversible two-state. Least square fitting of experimental andmodels were done using Solver function of Excel software. Results showed that fitting parameters for two models are in very good agreement, but two extra parameters are obtained in Lumry-Eyring model, T1/2 and H.Lumry--Eyring model gives large difference between T1/2 and T* separating thermal effects of two steps. The low values of H and T1/2 cause that the thermal effects of first step (Equilibrium)of lumry-Eyring model to be omitted. This means that in low temperatures, the unfolded and fold states are in fast equilibrium.
| Item Type: | Conference or Workshop Item (Speech) |
|---|---|
| زبان سند : | انگلیسی |
| نویسنده اول : | مجتبی امانی |
| کلیدواژه ها (انگلیسی): | : DSC; Lumry-Eyring; fitting; Modeling; irreversible |
| Subjects: | QU Biochemistry |
| Divisions: | Faculty of Medicine > Department of Basic Sciences > Department of Biophysics |
| ID Code: | 8025 |
| Deposited By: | Dr Mojtaba Amani |
| Deposited On: | 14 Sep 1395 10:45 |
| Last Modified: | 14 Sep 1395 10:45 |
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