بیان زیرواحد B انتروتوکسین حساس به حرارت اشریشیاکلی انتروتوکسیژنیک در ساکارومایسس سروزیه

آهنگرزاده رضایی, محمد and رضایی, عباس and موذنی, سید محمد and هاتف سلمانیان, علی and یاسودا, یوکو and توچیکوبو, کونیو and نجار پیرایه, شهین and ارزنلو, محسن (1384) بیان زیرواحد B انتروتوکسین حساس به حرارت اشریشیاکلی انتروتوکسیژنیک در ساکارومایسس سروزیه. The Journal of Microbiology ــ 43 (4). pp. 354-360. شاپا 1225-8873

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Expression of Escherichia coli Heat-labile Enterotoxin B Subunit (LTB) in Saccharomyces cerevisiae

English Abstract

Heat-labile enterotoxin B subunit (LTB) of enterotoxigenic Escherichia coli (ETEC) is both a strong mucosal adjuvant and immunogen. It is a subunit vaccine candidate to be used against ETEC-induced diarrhea. It has already been expressed in several bacterial and plant systems. In order to construct yeast expressing vector for the LTB protein, the eltB gene encoding LTB was amplified from a human origin enterotoxigenic E. coli DNA by PCR. The expression plasmid pLTB83 was constructed by inserting the eltB gene into the pYES2 shuttle vector immediately downstream of the GAL1 promoter. The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. The LTB protein was detected in the total soluble protein of the yeast by SDS-PAGE analysis. Quantitative ELISA showed that the maximum amount of LTB protein expressed in the yeast was approximately 1.9% of the total soluble protein. Immunoblotting analysis showed the yeast-derived LTB protein was antigenically indistinguishable from bacterial LTB protein. Since the whole-recombinant yeast has been introduced as a new vaccine formulation the expression of LTB in S. cerevisiae can offer an inexpensive yet effective strategy to protect against ETEC, especially in developing countries where it is needed most

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محمد آهنگرزاده رضایی
نویسنده مسئول :محسن ارزنلو
Additional Information:0.967 : Impact Factor Abstracted/Indexed in: 1- Science Citation Index Expanded (SciSearch), PubMed/Medline, 2-SCOPUS, EMBASE, Chemical Abstracts Service (CAS), BIOSIS, 3-Google Scholar, CSA, CAB International, Academic OneFile, Biological Abstracts, Biotechnology Citation Index, EMBiology, Expanded Academic, Food Science and Technology Abstracts, Health Reference Center Academic, IBIDS, Journal Citation Reports/Science Edition, OCLC, SCImago, Summon by Serial Solutions
کلیدواژه ها (انگلیسی):enterotoxigenic Escherichia coli , LTB , Saccharomyces cerevisiae , gene expression , immunoblotting
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:815
Deposited By: MS Soghra Golmaghani
Deposited On:01 Oct 1388 10:24
Last Modified:24 Nov 1391 08:17

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