ترکیب اثر ضدآپوپتوز اریتروپویتین و ملاتونین بر آسیب کلیه ناشی از ایسکمی-رپرفیوژن در موشهای صحرایی

بنایی, شکوفه and احمدی اصل, ناصر and عالی همتی, علیرضا (1395) ترکیب اثر ضدآپوپتوز اریتروپویتین و ملاتونین بر آسیب کلیه ناشی از ایسکمی-رپرفیوژن در موشهای صحرایی. Acta Medica Iranica ــ 54 (10). pp. 624-630. شاپا 0044-6025

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Combination Anti-Apoptotic Effect of Erythropoietin and Melatonin on Ischemia Reperfusion-Induced Renal Injury in Rats

English Abstract

Renal ischemia-reperfusion (IR) contributes to the development of acute renal failure (ARF). Oxygen free radicals are considered to be principal components involved in the pathophysiological tissue alterations observed during renal IR. The purpose of this study was to investigate the combination effect of melatonin (MEL) and erythropoietin (EPO), which are a potent antioxidant and anti-apoptotic agents, in IR induced renal injury in rats. Wistar Albino rats were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 h reperfusion. MEL (10 mg/kg, i.p) and EPO (5000 U/kg, i.p) were administered prior to ischemia. After 24 h reperfusion, following decapitation, blood samples were collected for the determination of superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA) levels. Also, renal samples were taken for histological evaluation and apoptosis assay. Ischemia reperfusion increased SOD, GPx, MDA levels, and TUNEL positive cells. Histopathological findings of the IR group confirmed that there was renal impairment in the tubular epithelium. Treatment with EPO and MEL decreased SOD, GPx, and MDA levels, histopathological changes, and TUNEL positive cells. These results indicated that the combination of MEL and EPO could not exert more nephroprotective and anti-apoptotic effects than MEL treatment in renal ischemia-reperfusion injury.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :شکوفه بنایی
نویسنده :ناصر احمدی اصل
نویسنده :علیرضا عالی همتی
Additional Information:Indexed in: Pubmed, Scopus, EMBASE , Index Copernicus , DOAJ, Google Scholar , Magiran , Iranmedex, ISC
کلیدواژه ها (انگلیسی):Renal ischemia reperfusion; Apoptosis; Melatonin; Erythropoietin; Lipid peroxidation
Subjects:QT physiology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:8159
Deposited By: Dr shokofeh banaei kivi
Deposited On:11 Sep 1395 07:24
Last Modified:22 May 1399 07:16

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