عدم ارتباط میان چندریختی MNS16A در زیر واحد کاتالیتیک آنزیم تلومراز و سرطان پستان در اردبیل

عنوان انگليسی

No association between polymorphism MNS16A in the telomerase catalytic subunit gene (hTERT) and breast cancer in Ardabil, Iran

خلاصه انگلیسی

Breast cancer is the most frequent malignancy among women and its mortality is estimated to be about 519,000 deaths per year. The telomerase activity seems to be necessary for the immortality of tumor cells. The association between telomerase reactivation and its catalytic subunit's (hTERT) expression has been reported in several studies. The length polymorphism MNS16A in the hTERT gene's promoter has been recognized to be associated with its expression status. The aim of this study was assaying the role of this polymorphism in conferring susceptibility of gaining breast cancer in women of Ardabil. Among the women affected with breast cancer in Ardabil, 53 paraffin embedded tumor samples were selected. Fifty-one persons with any positive history of cancer in them and their 1st and 2nd degree of relatives have been selected as control group. After DNA extraction by phenol-chloroform based method, the presence of variants of the polymorphism MNS16A was detected by PCR method. The alleles lengthen 302 and 333 bp were nominated as L [Long] allele and S [Short] allele was referred to fragments with 243 and 272 bp lengths. Data was analyzed by statistical methods and P value less than 0.05 was determined as significant difference. The frequency of genotypes SS, SL, and LL among cases were 56.6%, 18.86%, and 24.52%, respectively, and were 50.98%, 24.49%, and 21.56% for genotypes SS, SL, and LL among control group. Any significant relationship was detected between cases and control groups [P>0.05]. In this investigation, it was revealed that the noted polymorphism seems not to be a susceptibility factor for gaining breast cancer in Ardabil province.

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