فعالیت تلومرازی سلولهای بنیادی مزانشیمی بندناف انسانی القا شده با سلنیم با سطح متفاوتی از بیان c-Myc و p53 همراه است

حسین زاده انور, لیلا and حسینی اصل, سید سعید and محمدزاده وردین, محمد and سقا, محسن (1395) فعالیت تلومرازی سلولهای بنیادی مزانشیمی بندناف انسانی القا شده با سلنیم با سطح متفاوتی از بیان c-Myc و p53 همراه است. DNA and Cell Biology ــ 36 (1). pp. 34-41. شاپا 1044-5498


Official URL: https://www.liebertpub.com/doi/10.1089/dna.2016.34...


The Telomerase Activity of Selenium-Induced Human Umbilical Cord Mesenchymal Stem Cells Is Associated With Different Levels of c-Myc and p53 Expression

English Abstract

Selenium—as a trace element—is nutritionally essential for humans. It prevents cancerous growth by inhibiting the telomerase activity but the mechanism involved in regulation of telomerase activity in normal telomerase-positive cells remains to be elucidated. Here, we find out whether the effect of sodium selenite and selenomethionine on telomerase activity in human umbilical cord-derived mesenchymal stem cells (hUCMSCs) is associated with different levels of c-Myc and p53 expression. The use of different staining methods including ethidium bromide/acridine orange and DAPI in addition to telomeric repeat amplification protocol assay and real-time PCR indicated that different forms of selenium have opposite impacts on c-Myc and p53 expressions in both hUCMSCs and AGS, a gastric adenocarcinoma cell line, as a positive control. Our findings suggest that the signaling pathways involved in the regulation of telomerase activity in malignant and normal telomerase-positive cell types are somewhat different, at least on the c-Myc and P53 expression levels.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :لیلا حسین زاده انور
نویسنده :سید سعید حسینی اصل
نویسنده :محمد محمدزاده وردین
نویسنده مسئول :محسن سقا
Additional Information:Impact Factor (2017) 2.634 Indexed in: ISI, PubMed/Medline, Scopus, Embase
کلیدواژه ها (انگلیسی):P53; c-Myc; hUCMSCs; selenium; telomerase activity
Subjects:QS Human Anatomy
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:8180
Deposited By: Dr Mohsen Sagha
Deposited On:11 Sep 1395 06:21
Last Modified:24 Jul 1400 14:09

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