title

تشخیص مستقیم تریپتوفان برای تشخیص سریع متاستاز سلول های سرطانی توسط حسگر فوق العاده حساس و بسیار گزینشی الکتروشیمیایی

مجیدی, میر رضا and کرمی, پری and جوهری اهر, محمد and امیدی, یداله (1395) تشخیص مستقیم تریپتوفان برای تشخیص سریع متاستاز سلول های سرطانی توسط حسگر فوق العاده حساس و بسیار گزینشی الکتروشیمیایی. Analytical Methods ــ 8 (44). pp. 7910-7919. شاپا 1759-9660

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Title

Direct detection of tryptophan for rapid diagnosis of cancer cell metastasis competence by an ultra-sensitive and highly selective electrochemical biosensor

English Abstract

The detection of L-tryptophan (Trp) in the extracellular matrix (ECM) of solid tumors is important, particularly in metastatic tumors, which catabolize Trp to kynurenine to escape from host immune system-mediated recognition. The presence of a co-existing amino acid such as L-tyrosine (Tyr) in the ECM routinely interferes with the detection of Trp. The current study demonstrates the development of aptamer-assisted ultra-sensitive and label free biosensor (aptasensor) based on the constant current-potentiometric striping analysis (CC-PSA) technique used for quantitative Trp analysis. To prepare the aptasensor, a gold electrode was first decorated with carboxylated multiwall carbon nanotubes (MWCNTs) and then armed with Trp aptamer molecules (Apt). The engineered aptasensor was characterized electrochemically by cyclic voltammetry (CV), linear sweep voltammetry (LSV), and CC-PSA. For this biosensor, the limit of detection (LOD) was found to be 6.4 × 10−11 M (S/N = 3) and two linear detection ranges (i.e. 1.0 × 10−10 to 1.0 × 10−5 and 1.0 × 10−5 to 3.0 × 10−4 M) were observed in the calibration graph. For proof-of-technology, the aptasensor was used for the detection of Trp in biological samples such as cow's milk and human blood serum, saliva, and urine samples. Taking a good facet of the proposed aptasensor into account, it was implemented for the detection of the Trp consumption rate in various human cancer cell lines such as HepG2 (hepatocarcinoma), 1321NI (astrocytoma), Calu-6 (lung carcinoma), NCI-H1299 (lung carcinoma), and HT29 (colorectal carcinoma).

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :میر رضا مجیدی
نویسنده :پری کرمی
نویسنده :محمد جوهری اهر
نویسنده مسئول :یداله امیدی
Additional Information:Impact Factor (2015) 1.915 Indexed in: ISI, Scopus, Chemical Abstracts
کلیدواژه ها (انگلیسی):tryptophan,rapid diagnosis,cancer cell metastasis,ultra-sensitive biosensor, electrochemical biosensor
Subjects:QV pharmacology > QV 744 Medicinal Chemistry
Divisions:School of Pharmacy > Department of Pharmaceutical Biomaterials
ID Code:8212
Deposited By: MR Mohammad Johari-Ahar
Deposited On:17 Sep 1395 05:39
Last Modified:16 Dec 1395 05:42

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