اثر محیط کشت بر پایداری شیمیایی و فعالیت ضد میکروبی آلیسین

ایمانی راد, حامد and ارزنلو, محسن and رنجبر امید, مهسا and رواجی, صابر and پیری دوگاهه, هادی (1395) اثر محیط کشت بر پایداری شیمیایی و فعالیت ضد میکروبی آلیسین. Journal of Functional Foods ــ 28 . pp. 321-325. شاپا 1756-4646

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Official URL: http://www.sciencedirect.com/science/journal/17564...


Effect of culture media on chemical stability and antibacterial activity of allicin

English Abstract

Minimum Inhibitory Concentration (MIC) of allicin were determined for 11 bacterial strains using 6 culture media including Todd Hewitt Broth (THB), Brain Heart Infusion (BHI) Broth, Tryptic Soy Broth (TSB), Mueller Hinton Broth (MHB), Luria Bertani (LB) Broth, and Brucella Broth (BB) at 37 °C. The chemical stability of allicin as a function of media was measured by HPLC. The MICs for most individual isolates grown at the same temperature varied with the media used. Higher MICs were obtained in BB, THB, BHI, TSB/MHB and LB with geometric mean values of 272.7, 145.2, 90.5, 77.3/72.6 and 46.7 μg ml−1 respectively. In media with higher MICs a big percent of allicin disappeared within the first 4 h of incubation and apparently allicin had shorter half-life compared to those with lower MIC values. The results showed that temperature directly influences the stability of allicin and shorter half-life was found at 37 °C. We conclude that the type of culture media, markedly influences the chemical stability of allicin and accordingly its MIC in a given bacterial species.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :حامد ایمانی راد
نویسنده مسئول :محسن ارزنلو
نویسنده :هادی پیری دوگاهه
Additional Information:Impact Factor(2015) 3.973 Indexed in: ISI, Scopus, Current Contents/Agriculture, Biology & Environmental Sciences, ScienceDirect, Scirus
کلیدواژه ها (انگلیسی):Allicin; Culture media; Antimicrobial activity; Minimum Inhibitory Concentration; Temperature; Chemical stability
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:8220
Deposited By: Dr Mohsen Arzanlou
Deposited On:20 Sep 1395 06:25
Last Modified:20 Sep 1395 06:25

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