تیمورپور, رقیه ، صادقیان, علی ، مشکات, زهرا ، اسمعیلی زاد, مجید ، سنکیان, مجتبی ، جباری, احمد رضا (1394) ساخت DNAواکسن کد کننده ژنهای Mtb32C and HBHA Genes از Mycobacterium tuberculosis. Jundishapur Journal of Microbiology ــ 8 (8). ص.ص.1-7. شاپا 2008-3645
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آدرس اینترنتی رسمی : http://jjmicrobiol.com
عنوان انگليسی
Construction of a DNA Vaccine Encoding Mtb32C and HBHA Genes of Mycobacterium tuberculosis
خلاصه انگلیسی
Background: Tuberculosis (TB) is a contagious disease caused by Mycobacterium tuberculosis. Development of a new vaccine for tuberculosis requires immunogenic antigens capable of inducing suitable and long-lasting T cell immunity. The emergence of multidrugs and extensively drug-resistant strains of M. tuberculosis has made it a global public health concern. Objectives: DNA vaccine is a straightforward method to introduce antigens to the host. In the present study, two immunodominant mycobacterial antigens (Mtb32C and HBHA) were isolated and cloned into pcDNA3.1 (+) to design and construct a new DNA vaccine. This vector is capable of producing new potent chimeric protein. Materials and Methods: Mtb32C (Rv0125) and heparin-binding haemagglutinin adhesion (HBHA) genes were amplified using polymerase chain reaction (PCR) of M. tuberculosis H37Rv genome and ligated into pcDNA3.1 (+). Colony-PCR and restriction enzyme analysis were used to confirm the accuracy of the cloning procedure. Results: In the current study, recombinant pcDNA3.1 (+) vector containing Mtb32C and HBHA genes was successfully constructed. The desired size of DNA fragment was observed using single and double digestion methods. Conclusions: Mtb32C and HBHA genes were successfully isolated from H37Rv genome and cloned into an eukaryotic expression vector. This vector can be considered as a vaccine to evaluate immune responses in animal models.
نوع سند : | مقاله |
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زبان سند : | انگلیسی |
نویسنده اول : | رقیه تیمورپور |
نویسنده مسئول : | زهرا مشکات |
ضریب تاثیر و نمایه مجلات: | Impact Factor(2015) 0.655 Indexed in: ISI, Pubmed/PMC, Scopus, Embase, EBSCO, DOAJ ,Google Scholar, ISC, SID, MagIran |
کلیدواژه ها (انگلیسی): | Vaccines, DNA; HBHA protein; Mtb32 antigen; Mycobacterium tuberculosis |
موضوعات : | QW میکروب شناسی و ایمنی شناسی |
بخش های دانشگاهی : | دانشكده پزشكي > گروه علوم پایه > بخش میکروبیولوژی |
کد شناسایی : | 8421 |
ارائه شده توسط : | دکتر رقیه تیمورپور |
ارائه شده در تاریخ : | 16 بهمن 1395 11:58 |
آخرین تغییر : | 06 مرداد 1399 09:37 |
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