ساخت DNAواکسن کد کننده ژنهای Mtb32C and HBHA Genes از Mycobacterium tuberculosis

تیمورپور, رقیه and صادقیان, علی and مشکات, زهرا and اسمعیلی زاد, مجید and سنکیان, مجتبی and جباری, احمد رضا (1394) ساخت DNAواکسن کد کننده ژنهای Mtb32C and HBHA Genes از Mycobacterium tuberculosis. Jundishapur Journal of Microbiology ــ 8 (8). pp. 1-7. شاپا 2008-3645


Official URL: http://jjmicrobiol.com


Construction of a DNA Vaccine Encoding Mtb32C and HBHA Genes of Mycobacterium tuberculosis

English Abstract

Background: Tuberculosis (TB) is a contagious disease caused by Mycobacterium tuberculosis. Development of a new vaccine for tuberculosis requires immunogenic antigens capable of inducing suitable and long-lasting T cell immunity. The emergence of multidrugs and extensively drug-resistant strains of M. tuberculosis has made it a global public health concern. Objectives: DNA vaccine is a straightforward method to introduce antigens to the host. In the present study, two immunodominant mycobacterial antigens (Mtb32C and HBHA) were isolated and cloned into pcDNA3.1 (+) to design and construct a new DNA vaccine. This vector is capable of producing new potent chimeric protein. Materials and Methods: Mtb32C (Rv0125) and heparin-binding haemagglutinin adhesion (HBHA) genes were amplified using polymerase chain reaction (PCR) of M. tuberculosis H37Rv genome and ligated into pcDNA3.1 (+). Colony-PCR and restriction enzyme analysis were used to confirm the accuracy of the cloning procedure. Results: In the current study, recombinant pcDNA3.1 (+) vector containing Mtb32C and HBHA genes was successfully constructed. The desired size of DNA fragment was observed using single and double digestion methods. Conclusions: Mtb32C and HBHA genes were successfully isolated from H37Rv genome and cloned into an eukaryotic expression vector. This vector can be considered as a vaccine to evaluate immune responses in animal models.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :رقیه تیمورپور
نویسنده مسئول :زهرا مشکات
Additional Information:Impact Factor(2015) 0.655 Indexed in: ISI, Pubmed/PMC, Scopus, Embase, EBSCO, DOAJ ,Google Scholar, ISC, SID, MagIran
کلیدواژه ها (انگلیسی):Vaccines, DNA; HBHA protein; Mtb32 antigen; Mycobacterium tuberculosis
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:8421
Deposited By: Dr Roghayeh Teimourpour
Deposited On:16 Nov 1395 11:58
Last Modified:06 May 1399 09:37

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