تیمورپور, رقیه and زارع, حسنا and رجب نیا, رمضان and یحیی پور, یوسف and مشکات, زهرا (1395) بررسی بیان یوکاریوتیک فیوژن ژن mtb32C-hbha از مایکوباکتریوم توبرکلوزیس در رده سلولی هپاتوکارسینوما. Iranian Journal of Microbiology ــ 8 (2). pp. 132-138. شاپا 2008-3289
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Title
Evaluation of the eukaryotic expression of mtb32C-hbha fusion gene of Mycobacterium tuberculosis in Hepatocarcinoma cell line
English Abstract
Background and Objectives: HBHA and Mtb32C have been isolated from culture supernatants of Mycobacterium tu- berculosis (M. tuberculosis) and Mycobacterium bovis (M. bovis) and their immunogenicity previously studies have been confirmed. In this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (Mt- b32C-HBHA), in producing new chimeric protein under the in-vitro condition was examined. Materials and Methods: In present study Huh7.5 cells was transfected with Mtb32C-HBHA –pCDNA3.1+ recombinant vector using the calcium phosphate method and expression of chimeric protein was assessed by RT-PCR and Western blot methods. Results: Results of RT-PCR and Western blot showed expression of 35.5 KD recombinant protein (Mtb32C-HBHA) in thiscell line. Conclusion: The constructed vector can produce two highly immunogenic antigens that fusion of them to gather makes chi- meric antigen with new traits. Other attempts are needed to evaluate specific properties of this new antigen such as molecular conformation modeling and immunologic characteristics in future studies.
Item Type: | Article |
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زبان سند : | انگلیسی |
نویسنده اول : | رقیه تیمورپور |
نویسنده مسئول : | زهرا مشکات |
Additional Information: | Indexed in: Pubmed/PMC, Scopus, EMBASE, CABI, EBSCO, Index Copernicus, DOAJ, Google Scholars, IranMedex, Magiran, SID, ISC. |
کلیدواژه ها (انگلیسی): | Mycobacterium tuberculosis, HBHA , Mtb32C, Huh7.5 cells |
Subjects: | QW Microbiology and Immunology |
Divisions: | Faculty of Medicine > Department of Basic Sciences > Department of Microbiology |
ID Code: | 8425 |
Deposited By: | Dr Roghayeh Teimourpour |
Deposited On: | 18 Oct 1395 11:56 |
Last Modified: | 06 May 1399 09:33 |
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