بررسی بیان یوکاریوتیک فیوژن ژن mtb32C-hbha از مایکوباکتریوم توبرکلوزیس در رده سلولی هپاتوکارسینوما

تیمورپور, رقیه and زارع, حسنا and رجب نیا, رمضان and یحیی پور, یوسف and مشکات, زهرا (1395) بررسی بیان یوکاریوتیک فیوژن ژن mtb32C-hbha از مایکوباکتریوم توبرکلوزیس در رده سلولی هپاتوکارسینوما. Iranian Journal of Microbiology ــ 8 (2). pp. 132-138. شاپا 2008-3289


Official URL: http://ijm.tums.ac.ir


Evaluation of the eukaryotic expression of mtb32C-hbha fusion gene of Mycobacterium tuberculosis in Hepatocarcinoma cell line

English Abstract

Background and Objectives: HBHA and Mtb32C have been isolated from culture supernatants of Mycobacterium tu- berculosis (M. tuberculosis) and Mycobacterium bovis (M. bovis) and their immunogenicity previously studies have been confirmed. In this study, capability of constructed vector containing two mycobacterial immunodaminant antigens (Mt- b32C-HBHA), in producing new chimeric protein under the in-vitro condition was examined. Materials and Methods: In present study Huh7.5 cells was transfected with Mtb32C-HBHA –pCDNA3.1+ recombinant vector using the calcium phosphate method and expression of chimeric protein was assessed by RT-PCR and Western blot methods. Results: Results of RT-PCR and Western blot showed expression of 35.5 KD recombinant protein (Mtb32C-HBHA) in thiscell line. Conclusion: The constructed vector can produce two highly immunogenic antigens that fusion of them to gather makes chi- meric antigen with new traits. Other attempts are needed to evaluate specific properties of this new antigen such as molecular conformation modeling and immunologic characteristics in future studies.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :رقیه تیمورپور
نویسنده مسئول :زهرا مشکات
Additional Information:Indexed in: Pubmed/PMC, Scopus, EMBASE, CABI, EBSCO, Index Copernicus, DOAJ, Google Scholars, IranMedex, Magiran, SID, ISC.
کلیدواژه ها (انگلیسی):Mycobacterium tuberculosis, HBHA , Mtb32C, Huh7.5 cells
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:8425
Deposited By: Dr Roghayeh Teimourpour
Deposited On:18 Oct 1395 11:56
Last Modified:06 May 1399 09:33

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