طراحی و ساخت DNA واکسن کد کننده ژنTb10.4از مایکوباکتریوم توبرکلوزیس

رشیدیان, سمیرا and تیمورپور, رقیه and مشکات, زهرا (1395) طراحی و ساخت DNA واکسن کد کننده ژنTb10.4از مایکوباکتریوم توبرکلوزیس. Iranian journal of pathology ــ 11 (2). pp. 112-119. شاپا 1735-5303


Official URL: http://ijp.iranpath.org


Designing and Construction of a DNA Vaccine Encoding Tb10.4 Gene of Mycobacterium tuberculosis

English Abstract

Background: Tuberculosis (TB) remains as a major cause of death. Constructionof a new vaccine against tuberculosis is an effective way to control it. Several vaccinesagainst this disease have been developed. The aim of the present study was to cloningof tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryoticcells.Methods: Firstly, tb10.4 fragment was amplified by PCR and the PCR productwas digested with restriction enzymes. Next, it was cloned into pcDNA3.1+ plasmid.Following that, pcDNA3.1+/tb10.4 recombinant plasmid was transfected into eukaryotic cells.Results: 5700 bp band for pcDNA3.1+/tb10.4 recombinant plasmid and 297 bp fragment for tb10.4 were observed. Cloning and transfection were successful and designed recombinant vector was confirmed by sequencing. Conclusion: Successful cloning provides a basis for the development of new DNA vaccines against tuberculosis

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :سمیرا رشیدیان
نویسنده :رقیه تیمورپور
نویسنده مسئول :زهرا مشکات
Additional Information:Indexed in: Pubmed/PMC, Scopus, ISC, DOAJ, EBSCO, magiran, Google Scholar, CINAHL,
کلیدواژه ها (انگلیسی):Mycobacterium tuberculosis; tb10.4; DNA vaccine
Subjects:QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Microbiology
ID Code:8426
Deposited By: Dr Roghayeh Teimourpour
Deposited On:22 Oct 1395 08:14
Last Modified:06 May 1399 09:34

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