رشیدیان, سمیرا and تیمورپور, رقیه and مشکات, زهرا (1395) طراحی و ساخت DNA واکسن کد کننده ژنTb10.4از مایکوباکتریوم توبرکلوزیس. Iranian journal of pathology ــ 11 (2). pp. 112-119. شاپا 1735-5303
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Official URL: http://ijp.iranpath.org
Title
Designing and Construction of a DNA Vaccine Encoding Tb10.4 Gene of Mycobacterium tuberculosis
English Abstract
Background: Tuberculosis (TB) remains as a major cause of death. Constructionof a new vaccine against tuberculosis is an effective way to control it. Several vaccinesagainst this disease have been developed. The aim of the present study was to cloningof tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryoticcells.Methods: Firstly, tb10.4 fragment was amplified by PCR and the PCR productwas digested with restriction enzymes. Next, it was cloned into pcDNA3.1+ plasmid.Following that, pcDNA3.1+/tb10.4 recombinant plasmid was transfected into eukaryotic cells.Results: 5700 bp band for pcDNA3.1+/tb10.4 recombinant plasmid and 297 bp fragment for tb10.4 were observed. Cloning and transfection were successful and designed recombinant vector was confirmed by sequencing. Conclusion: Successful cloning provides a basis for the development of new DNA vaccines against tuberculosis
| Item Type: | Article |
|---|---|
| زبان سند : | انگلیسی |
| نویسنده اول : | سمیرا رشیدیان |
| نویسنده : | رقیه تیمورپور |
| نویسنده مسئول : | زهرا مشکات |
| Additional Information: | Indexed in: Pubmed/PMC, Scopus, ISC, DOAJ, EBSCO, magiran, Google Scholar, CINAHL, |
| کلیدواژه ها (انگلیسی): | Mycobacterium tuberculosis; tb10.4; DNA vaccine |
| Subjects: | QW Microbiology and Immunology |
| Divisions: | Faculty of Medicine > Department of Basic Sciences > Department of Microbiology |
| ID Code: | 8426 |
| Deposited By: | Dr Roghayeh Teimourpour |
| Deposited On: | 22 Oct 1395 08:14 |
| Last Modified: | 06 May 1399 09:34 |
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