آلفاتوکوفرول ادم مغزی را کاهش داده و از سد خونی - مغزی پس از وقوع ایسکمی موضعی مغز محافظت می کند(2017)

حق نژاد آذر, عادل and عریان, شهربانو and بهلولی, شهاب and پناهپور, حمداله (1395) آلفاتوکوفرول ادم مغزی را کاهش داده و از سد خونی - مغزی پس از وقوع ایسکمی موضعی مغز محافظت می کند(2017). Medical Principles and Practice ــ 26 (1). pp. 17-22. شاپا 1011-7571

Text - Published Version

Official URL: https://www.karger.com/Journal/Home/224259


Alpha-Tocopherol Reduces Brain Edema and Protects Blood-Brain Barrier Integrity following Focal Cerebral Ischemia in Rats

English Abstract

Objective: This study was conducted to examine the neuroprotective effects of α-tocopherol against edema formation and disruption of the blood-brain barrier (BBB) following transient focal cerebral ischemia in rats. Materials and Methods: Ninety-six male Sprague-Dawley rats were divided into 3 major groups (n = 32 in each), namely the sham, and control and α-tocopherol-treated (30 mg/kg) ischemic groups. Transient focal cerebral ischemia (90 min) was induced by occlusion of the left middle cerebral artery. At the end of the 24-hour reperfusion period, the animals were randomly selected and used for 4 investigations (n = 8) in each of the 3 main groups: (a) assessment of neurological score and measurement of infarct size, (b) detection of brain edema formation by the wet/dry method, (c) evaluation of BBB permeability using the Evans blue (EB) extravasation technique, and (d) assessment of the malondialdehyde (MDA) and reduced glutathione (GSH) concentrations using high-performance liquid chromatography methods. Results: Induction of cerebral ischemia in the control group produced extensive brain edema (brain water content 83.8 ± 0.11%) and EB leakage into brain parenchyma (14.58 ± 1.29 µg/g) in conjunction with reduced GSH and elevated MDA levels (5.86 ± 0.31 mmol/mg and 63.57 ± 5.42 nmol/mg, respectively). Treatment with α-tocopherol significantly lowered brain edema formation and reduced EB leakage compared with the control group (p < 0.001, 80.1 ± 0.32% and 6.66 ± 0.87 µg/g, respectively). Meanwhile, treatment with α-tocopherol retained tissue GSH levels and led to a lower MDA level (p < 0.01, 10.17 ± 0.83 mmol/mg, and p < 0.001, 26.84 ± 4.79 nmol/mg, respectively). Conclusion: Treatment with α-tocopherol reduced ischemic edema formation and produced protective effects on BBB function following ischemic stroke occurrence. This effect could be through increasing antioxidant activity

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :عادل حق نژاد آذر
نویسنده :شهاب بهلولی
نویسنده مسئول :حمداله پناهپور
Additional Information:Impact Factor(2016) 1.469 Indexed in: ISI, Pubmed, Medline/Inde Medicus, Scopus, Google Scholar, Embase, CINAHL Complete, WorldCat
کلیدواژه ها (انگلیسی):α-Tocopherol, Cerebral ischemia, Brain edema, Malondialdehyde, Glutathione
Subjects:QT physiology
QV pharmacology
WL Nervous system
WT Geriatrics . Chronic Diseases
Divisions:School of Pharmacy > Department of Clinical Pharmacy
ID Code:8452
Deposited By: Dr Hamdollah Panahpour
Deposited On:05 Nov 1395 11:32
Last Modified:13 Sep 1396 07:06

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