تولید بهینه عصاره توموری از سلول های گلیال B92

رحمانی کوکیا, نسیم and دلیرژ, نوروز and علی پناه مقدم, رضا (1395) تولید بهینه عصاره توموری از سلول های گلیال B92. در: 13th International Congress of Immunology and Allergy of Iran, Apr 26-29, 2016, Tabriz - Iran.


Official URL: http://www.icia.ir


Optimized Production of Tumor Cell Lysates from B92 Glial cells

English Abstract

Introduction: Lysates from tumor cells are reported to induce maturation of dendritic cells (DCs) and are used in clinical settings for DC-based vaccination against tumors. Further studies showed that tumor lysates consist of HSP-peptide complexes (HSP-PC) that their expression increases in hyperthermia. This survey was designed to offer an efficient procedure for inducing the maximum amount of glial tumor cell lysates. Materials and Methods: Tumor cell line, B92 was cultured in RPMI supplemented with 10% FBS.To apply heat stress, culture flasks were sealed and immersed for 90 min in a water bath at 43°C, allowed to recover at 37°C for 8 h in a 5% CO 2 incubator. Then, cells were washed in condition medium and subjected to four freeze and thaw cycles to obtain a crude lysate. Results: Protein determination by the Bradford method showed a high level of the protein concentration in tumor lysate after exposure to heat. Conclusion: The present study proposes a valid method for generating of B92 rat tumor cell lysates consist of HSP-PCfrom cancer cells that form effective bases for immunotherapy approaches with unique properties.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :نسیم رحمانی کوکیا
نویسنده :نوروز دلیرژ
نویسنده :رضا علی پناه مقدم
کلیدواژه ها (انگلیسی):optimized production, tumor cell lysate, Hsp-pc
Subjects:QU Biochemistry
QW Microbiology and Immunology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
ID Code:8462
Deposited By: Dr Reza Alipanah-Moghadam
Deposited On:01 Dec 1395 09:08
Last Modified:01 Dec 1395 09:08

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