چه اطلاعاتی از تغییر سرعت روبش در کالریمتری روبش افتراقی می توان بدست آورد؟

امانی, مجتبی and موسوی موحدی, علی اکبر and Kurganov, Boris I (1396) چه اطلاعاتی از تغییر سرعت روبش در کالریمتری روبش افتراقی می توان بدست آورد؟. International Journal of Biological Macromolecules ــ 99 . 151 - 159. شاپا 0141-8130

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Official URL: http://www.sciencedirect.com/science/journal/01418...


What can we get from varying scan rate in protein differential scanning calorimetry

English Abstract

Differential scanning calorimetry has many advantages over other techniques to study the thermal stability of proteins due to its direct measurement of thermodynamic parameters. Most proteins undergo irreversible thermal denaturation causing their thermogram to be scan rate dependent. We modeled reversible and irreversible protein thermograms at varying scan rates. The complete Lumry-Eyring model was used to model the irreversible thermograms at various values of T1/2 (temperature at which equilibrium constant equals unity) and T* (temperature at which rate constant equals 1 min�1). Our results have shown that the thermal effects of two processes are integrated with decreasing the T* relative to T1/2. It is also shown that the shape of second derivatives of thermograms under different conditions have specific pattern which can be used to judge and estimate the correct model for protein denaturation.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :مجتبی امانی
نویسنده :علی اکبر موسوی موحدی
نویسنده :Boris I Kurganov
Additional Information:Impact Factor (2015) 3.138 Indexed in: ISI, Pubmed/Medline/Index Medicus, scopus, Elsevier BIOBASE, Current Contents, EMBASE, EMBiology
کلیدواژه ها (انگلیسی):Differential scanning calorimetry; Second derivative; Irreversible thermal denaturation; Modeling; Scan rate
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:8553
Deposited By: Dr Mojtaba Amani
Deposited On:14 Dec 1395 10:15
Last Modified:14 Dec 1395 10:15

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