تهیه و ارزیابی درون تن فرمولاسیون آهسته رهش نانوکامپوزیت تریپاراتاید با استفاده از نانوذرات پخش شده در بستر هیدروژل (هیالورونیک اسید- جفامین)

بهاری جوان, نیکا and منتظری, حامد and رضایی شیرمرد, لیلا and جعفری امید, نرسی and بربری, غلام رضا and امینی, محسن and قهرمانی, محمدحسین and رفیعی تهرانی, مرتضی and عابدین درکوش, فرید (1396) تهیه و ارزیابی درون تن فرمولاسیون آهسته رهش نانوکامپوزیت تریپاراتاید با استفاده از نانوذرات پخش شده در بستر هیدروژل (هیالورونیک اسید- جفامین). European Journal of Pharmaceutical Sciences ــ 101 . pp. 167-181. شاپا 0928-0987

Text - Published Version

Official URL: http://www.sciencedirect.com/science/journal/09280...


Preparation, characterization and in vivo evaluation of a combination delivery system based on hyaluronic acid/jeffamine hydrogel loaded with PHBV/PLGA blend nanoparticles for prolonged delivery of Teriparatide

English Abstract

In the current study, biodegradable PHBV/PLGA blend nanoparticles (NPs) containing Teriparatide were loaded in hyaluronic acid/jeffamine (HA-JEF ED-600) hydrogel to prepare a combination delivery system (CDS) for prolonged delivery of Teriparatide. The principal purpose of the present study was to formulate an effective and prolonged riparatide delivery system in order to reduce the frequency of injection and thus enhance patient's compliance. Morphological properties, swelling behaviour, crosslinking efficiency and rheological characterization of HA-JEF ED-600 hydrogel were evaluated. The CDS was acquired by adding PHBV/PLGA NPs to HA-JEF ED-600 hydrogel simultaneously with crosslinking reaction. The percentage of NPs incorporation within the hydrogel as well as the loading capacity and morphology of Teriparatide loaded CDS were examined. Intrinsic fluorescence and circular dichroism spectroscopy proved that Teriparatide remains stable after processing. The release profile represented 63% Teriparatide release from CDS within 50 days with lower burst release compared to NPs and hydrogel. MTT assay was conducted by using NIH3T3 cell line and no sign of reduction in cell viability was observed. Based on Miller and Tainter method, LD50 of Teriparatide loaded CDS was 131.8 mg/kg. In vivo studies demonstrated that Teriparatide loaded CDS could effectively increase serum calcium level after subcutaneous injection in mice. Favourable results in the current study introduced CDS as a promising candidate for controlled delivery of Teriparatide and pave the way for future investigations in the field of designing prolonged delivery systems for other peptides and proteins.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :نیکا بهاری جوان
نویسنده :لیلا رضایی شیرمرد
نویسنده مسئول :فرید عابدین درکوش
Additional Information:Impact Factor:(2015) 3.773 indexed in: ISI , Pubmed/Medline/Index Medicus, Scopus, Chemical Abstracts, BIOSIS, Reaxys, Current Contents/Life Sciences, International Pharmaceutical Abstracts, EMBASE,CAB Abstracts
کلیدواژه ها (انگلیسی):Combination delivery system; PHBV/PLGA blend nanoparticles , Hyaluronic acid/jeffamine hydrogel , Prolonged delivery , Teriparatide
Subjects:QV pharmacology > QV 704 Pharmaceutics
Divisions:School of Pharmacy > Department of Pharmaceutics
ID Code:8579
Deposited By: دکتر لیلا رضایی
Deposited On:15 Jan 1396 09:10
Last Modified:06 Jun 1397 13:36

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