title

اثر اسمولیتی سوکروز بر تقلیب حرارتی آمین اکسیداز حاوی مس از جوانه نخود

امانی, مجتبی and برزگر, ابوذر and مأذنی, محمد (1396) اثر اسمولیتی سوکروز بر تقلیب حرارتی آمین اکسیداز حاوی مس از جوانه نخود. The Protein Journal ــ 36 (2). pp. 147-153. شاپا 1572-3887

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Title

Osmolytic Effect of Sucrose on Thermal Denaturation of Pea Seedling Copper Amine Oxidase

English Abstract

Protein stability is a subject of interest by many researchers. One of the common methods to increase the protein stability is using the osmolytes. Many studies and theories analyzed and explained osmolytic effect by equilibrium thermodynamic while most proteins undergo an irreversible denaturation. In current study we investigated the effect of sucrose as an osmolyte on the thermal denaturation of pea seedlings amine oxidase by the enzyme activity, fluorescence spectroscopy, circular dichroism, and differential scanning calorimetry. All experiments are in agreement that pea seedlings amine oxidase denaturation is controlled kinetically and its kinetic stability is increased in presence of sucrose. Differential scanning calorimetry experiments at different scanning rates showed that pea seedlings amine oxidase unfolding obeys two-state irreversible model. Fitting the differential scanning calorimetry data to two-state irreversible model showed that unfolding enthalpy and T *, temperature at which rate constant equals unit per minute, are increased while activation energy is not affected by increase in sucrose concentration. We concluded that osmolytes decrease the molecular oscillation of irreversible proteins which leads to decline in unfolding rate constant.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :مجتبی امانی
نویسنده :ابوذر برزگر
نویسنده :محمد مأذنی
Additional Information:Impact Factor(2015) 1.029 Indexed in: ISI, Pubmed/Medline/Index Medicus, Scopus, Chemical Abstracts Service (CAS), Google Scholar, EBSCO Discovery Service, CSA, CAB International, Academic OneFile, Academic Search, ASFA, Biological Abstracts, BIOSIS, CAB Abstracts, ChemWeb, CSA Environmental Sciences, Current , Contents/ Life Sciences, Elsevier Biobase, EMBiology, Gale, Global Health
کلیدواژه ها (انگلیسی):Pea seedlings amine oxidase, Sucrose, Differential scanning calorimetry, Stability
Subjects:QU Biochemistry
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biophysics
ID Code:8602
Deposited By: Dr Mojtaba Amani
Deposited On:15 Jan 1396 10:02
Last Modified:03 Mar 1396 05:44

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