تاثیر ایتکوباسیون اپی تلیوم و ایزوپرنالین بر روی پاسخ دهی تراشه خوکچه هندی نسبت به متاکولین

بسکابادی, محمد حسین and اصلانی, محمدرضا and طباطبایی, آذردخت (1384) تاثیر ایتکوباسیون اپی تلیوم و ایزوپرنالین بر روی پاسخ دهی تراشه خوکچه هندی نسبت به متاکولین. Pharmacology ــ 76 (1). pp. 1-7. شاپا 0031-7012


Official URL: https://www.karger.com/Article/Abstract/88739


Influence of Epithelium and Isoprenaline Incubation on Responsiveness of Guinea-Pig Trachea to Methacholine

English Abstract

There are reports regarding harmful effect of long-term use of β2 -agonist drugs on asthma severity and airway responsiveness. In the present study, the responses of guinea pig trachea with intact and denuded epithelium (groups 1 and 2, n = 10) to methacholine as EC50 were measured in tissues nonincubated or incubated with 10 µmol/l isoprenaline during the resting period. The same protocol was performed in groups 3 and 4 (n = 5 for each group) with an additional 30 min rest time after isoprenaline incubation. The response of trachea with denuded epithelium (groups 2 and 4) to methacholine was significantly higher than that with intact epithelium both in incubated and nonincubated conditions (groups 1 and 3, p<0.05 to p < 0.001). Incubation with isoprenaline caused a significant reduction in the tracheal response to methacholine in both the denuded groups (p<0.005 and p<0.001) and intact epithelium groups (p<0.005 for both cases). The reduction in tracheal responsiveness to methacholine due to incubation in epithelium denuded trachea (groups 2 and 4) was non significantly greater than that of intact epithelium tissues. There was no difference between groups 3 and 4 with those of groups 1 and 2 in both incubated and non incubated conditions. The maximum contractility response to methacholine was not different between tracheal chains with denuded and intact epithelium and did not change due to incubation with isoprenaline. The results of this study indicate reduction of tracheal response to methacholine due to incubation of tissues with isoprenaline, which was relatively more pronounced in epithelium denuded trachea.

Item Type:Article
زبان سند : انگلیسی
نویسنده مسئول :محمد حسین بسکابادی
نویسنده :محمدرضا اصلانی
Additional Information:IF:1.24 Indexed in: ISI, PubMed/Medline, Scopus, Embase
کلیدواژه ها (انگلیسی):Epithelium , Adrenergic receptors , Asthma , Airway responsiveness , Desensitization
Subjects:QT physiology
QV pharmacology
WF Respiratory System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:8812
Deposited By: Dr Mohammad Reza Aslani
Deposited On:28 May 1396 05:11
Last Modified:26 Jun 1402 14:55

Repository Staff Only: item control page

Document Downloads

More statistics for this item...