اثرات تعاملی ضد التهابی سلولهای بینادی مزانشیمالی بر روی تغییرات آسمی القا شده بوسیله اوآلبومین در رتهای ویستار نر

احمدی, مهدی and رهبر قاضی, رضا and سلطانی, سینا and اصلانی, محمدرضا and کیهان منش, رعنا (1395) اثرات تعاملی ضد التهابی سلولهای بینادی مزانشیمالی بر روی تغییرات آسمی القا شده بوسیله اوآلبومین در رتهای ویستار نر. Inflammation ــ 39 (6). pp. 1960-1971. شاپا 0360-3997


Official URL: https://link.springer.com/article/10.1007/s10753-0...


Contributory Anti-Inflammatory Effects of Mesenchymal Stem Cells, Not Conditioned Media, On Ovalbumin-Induced ASthmatic Changes in Male Rats

English Abstract

Our aim in selecting an appropriate cell fraction and nditioned media (CM) was to achieve the suitable candidate for ameliorating long-term chronic asthmatic changes of respiratory tract. Thirty-six rats were classified into healthy and sensitized groups, which were further divided into three subgroups; rats received systemically 50 μl volume of PBS, CM, or 2 × 106 rat bone marrow-derived mesenchymal stem cells (rBMMSCs). Tracheal responsiveness (TR), immunologic responses, and recruitment of rBMMSCs into the lungs were evaluated. A high degree of TR and total WBC and percentages of eosinophils and neutrophils was significantly recorded in all sensitized groups rather than of controls (p < 0.001 to p < 0.05). Concurrently, a significant improvement of TR and eosinophil and neutrophil return toward normal levels was evident in sensitized rats receiving cells as compared to parallel asthmatic animals. Flow cytometric monitoring of lymphocyte subpopulation vealed a decrease in the number of CD3+CD4+ and concurrent increase in CD3+CD8+ in all sensitized rats as compared to control (p < 0.001 to p < 0.05). Noticeably, no significant modulatory effects of either cell or CM administration were achieved on the CD3+CD4+ and CD3+CD8+ populations in non-asthmatic rats. Corroborating our results, the number of CD3+CD4+ tended to increase (p < 0.05) which coincided with a decreased manner of CD3+CD8+ populations as compared to other asthmatic groups (p < 0.01 to p < 0.05).Moreover, stemcells could efficiently transmigrate to the lung parenchyma, albeit the dynamic of asthmatic changes stimulated the rate of recruited cells. Our study shed light on superior effects of mesenchymal stem cells, but not CM, in attenuating chronic asthmatic changes in the model of rat.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مهدی احمدی
نویسنده :رضا رهبر قاضی
نویسنده :سینا سلطانی
نویسنده :محمدرضا اصلانی
نویسنده مسئول :رعنا کیهان منش
Additional Information:Impact Factor(2016) 2.955 Indexed in: ISI, Pubmed/Medline/Index Medicus, Scopus, Chemical Abstracts Service (CAS), EMBASE, Google Scholar, EBSCO Discovery Service, CSA, CAB International, Academic OneFile, Academic Search, Biological Abstracts, BIOSIS, CAB Abstracts, CSA Environmental Sciences, Current Contents/ Life Sciences, Elsevier Biobase, EMBiology, Expanded Academic
کلیدواژه ها (انگلیسی):asthma; inflammation; conditioned media; mesenchymal stem cells; lymphocyte.
Subjects:QT physiology
QV pharmacology
WF Respiratory System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:8823
Deposited By: Dr Mohammad Reza Aslani
Deposited On:18 Apr 1396 08:15
Last Modified:18 Apr 1396 09:30

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