title

افزایش بیان ویسفاتین است همراه با میزان بیان فاکتور رونویسی NF-kB در تراشه رتهای نر نژاد ویستار چاقی حساس شده با اوآلبومین

اصلانی, محمدرضا and کیهان منش, رعنا and علیپور, محمد رضا (1396) افزایش بیان ویسفاتین است همراه با میزان بیان فاکتور رونویسی NF-kB در تراشه رتهای نر نژاد ویستار چاقی حساس شده با اوآلبومین. Medical Principles and Practice ــ 26 (4). pp. 351-358. شاپا 1011-7571

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Title

Increased Visfatin Expression Is Associated with Nuclear Factor-κB in Obese Ovalbumin-Sensitized Male Wistar Rat Tracheae

English Abstract

Objective: To investigate the effects of diet-induced obesity on the expression of nuclear factor-κB (NF-κB) and visfatin messenger RNA in male Wistar rats’ tracheae after sensitization with ovalbumin (OVA). Materials and Methods: Twenty male Wistar rats were divided into 4 groups ( n = 5 for each group), which included a control group fed a normal diet (ND) and groups fed normal diet, OVA-sensitized (S+ND); high-fat diet (HFD) only (diet-induced obesity); and high-fat diet, OVA-sensitized (S+HFD). All animals were fed for 8 weeks with standard chow or a high-fat diet, and then were sensitized and challenged with OVA or saline for another 4 weeks as per the above groups. The rats were anesthetized, after which the necks were exposed and the tracheae iso-lated and examined for expression levels of NF-κB and visfatin mRNA with the real-time polymerase chain reaction method. Data were compared between the different groups using one-way analysis of variance. Results: The expression level of NF-κB mRNA in the S+HFD group was 2.67, which was statistically higher than the levels in the ND (0.96; p = 0.001), S+ND (1.86; p = 0.05), and HFD (1.26; p = 0.001) groups. Also, the visfatin mRNA expression level in the S+HFD group was 4.21, which was higher than the levels in the ND (0.92), S+ND (1.79), and HFD (2.20) ( p = 0.001) groups. Conclusion: In this study, the expression levels of NF-κB and visfatin were markedly higher in the S+HFD group in comparison to the other groups. These findings indicate that alternative signaling pathways might be activated in diet-induced obesity associated with the OVA-sensitized animal model and could be responsible for possible altered sensitization phenotype.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :محمدرضا اصلانی
نویسنده :رعنا کیهان منش
نویسنده مسئول :محمد رضا علیپور
Additional Information:IF:1.469 Indexed in: ISI, PubMed/Medline, Scopus, Embase
کلیدواژه ها (انگلیسی):Obesity ,Asthma , Nuclear factor-κB, Visfatin
Subjects:QT physiology
WD Nutrition Disease and metabolic diseases
WF Respiratory System
WK Endocrine System
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of physiology
ID Code:8841
Deposited By: Dr Mohammad Reza Aslani
Deposited On:10 Apr 1396 09:39
Last Modified:26 Jun 1402 13:18

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