مقایسه شش روش برای استخراج DNA ریبوزومی و میتوکندریایی نماتودهای توکسوکارا و توکساسکاریس

میکائیلی, فتانه and کیا, عشرت بیگم and شربت خوری, میترا and شریف دینی, میثم and جلالی زند, ن and حیدری, زهرا and زارعی, ذبیح اله and Stensvold, C.R. and میرهندی, حسین (1392) مقایسه شش روش برای استخراج DNA ریبوزومی و میتوکندریایی نماتودهای توکسوکارا و توکساسکاریس. Experimental Parasitology ــ 134 (2). pp. 155-159. شاپا 0014-4894


Official URL: http://www.sciencedirect.com/science/article/pii/S...


Comparison of six simple methods for extracting ribosomal and mitochondrial DNA from Toxocara and Toxascaris nematodes

English Abstract

Six simple methods for extraction of ribosomal and mitochondrial DNA from Toxocara canis, Toxocara cati and Toxascaris leonina were compared by evaluating the presence, appearance and intensity of PCR products visualized on agarose gels and amplified from DNA extracted by each of the methods. For each species, two isolates were obtained from the intestines of their respective hosts: T. canis and T. leonina from dogs, and T. cati from cats. For all isolates, total DNA was extracted using six different methods, including grinding, boiling, crushing, beating, freeze–thawing and the use of a commercial kit. To evaluate the efficacy of each method, the internal transcribed spacer (ITS) region and the cytochrome c oxidase subunit 1 (cox1) gene were chosen as representative markers for ribosomal and mitochondrial DNA, respectively. Among the six DNA extraction methods, the beating method was the most cost effective for all three species, followed by the commercial kit. Both methods produced high intensity bands on agarose gels and were characterized by no or minimal smear formation, depending on gene target; however, beating was less expensive. We therefore recommend the beating method for studies where costs need to be kept at low levels.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :فتانه میکائیلی
نویسنده :عشرت بیگم کیا
نویسنده :میترا شربت خوری
نویسنده :میثم شریف دینی
نویسنده :ن جلالی زند
نویسنده :زهرا حیدری
نویسنده :ذبیح اله زارعی
نویسنده :C.R. Stensvold
نویسنده مسئول :حسین میرهندی
Additional Information:Impact Factor (2013) 1.859 Indexed in: ISI, PubMed/Medline/Index Medicus, Scopus
کلیدواژه ها (انگلیسی):Nematode; Toxocara; Toxascaris; DNA extraction; PCR
Subjects:QX Parasitology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:8843
Deposited By: Dr Zahra Heidari
Deposited On:12 May 1396 06:26
Last Modified:14 Oct 1400 09:05

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