title

توزیع سنی و جنسی دیروفیلاریاایمیتیس در میان سگ های مشکین شهر، شمال غرب ایران و آنالیز مولکولی سویه ها بر اساس ژن COX1

زارعی, ذبیح الله and کیا, عشرت بیگم and حیدری, زهرا and میکائیلی, فتانه and محبعلی, مهدی and شریف دینی, میثم (1395) توزیع سنی و جنسی دیروفیلاریاایمیتیس در میان سگ های مشکین شهر، شمال غرب ایران و آنالیز مولکولی سویه ها بر اساس ژن COX1. Veterinary Research Forum ــ 7 (4). pp. 329-334. شاپا 2008-8140

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Title

Age and sex distribution of Dirofilaria immitis among dogs in Meshkin-Shahr, northwest Iran and molecular analysis of the isolates based on COX1 gene

English Abstract

Dirofilaria immitis is an important filarial nematode in dogs. In this study, age and sex distribution of this zoonotic nematode among dogs were investigated in northwest of Iran in Meshkin-Shahr city. Molecular characteristics of the isolates, based on cytochrome oxidase subunit 1 (COX1) gene were compared to the isolates from other areas of the world.Blood samples were collected from 91 dogs which were selected by simple classified accidental sampling. Thin and thick blood smear examinations were used to find out infectivity with D. immitis. DNA extraction was performed from adult D. immitis recovered from heart of infected dogs. The COX1 gene was amplified and sequenced. Phylogenetic analysis was carried out using sequences obtained in this study along with relevant sequences deposited in the GenBank. Phylogenetic analysis and sequence variation was performed using MEGA software in comparison with those COX1 sequences deposited in GenBank. Out of 91 dogs, 19 (20.87%) were found positive for infection with D. immitis. There was no statistically significant difference between males and females of dogs in terms of D. immitis infection. However, the rate of infection in dogs more than 2 years old was significantly higher than those with lower age. Both sequences analyzed in this study showed 100% homology to each other. Intra-species variation of these isolates with those from other areas of the world amounted to 0 to 0.50%. Phylogenetic analysis of the COX1 gene suggested that it is conserved, and can be used for study on genetic diversity and classification of filarial nematodes

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :ذبیح الله زارعی
نویسنده :زهرا حیدری
نویسنده مسئول :میثم شریف دینی
Additional Information:Indexed in: ESCI, Pubmed/PMC, DOAJ, ISC, SID, Magiran, Google Scholar, Index Copernicus
کلیدواژه ها (انگلیسی):Dirofilaria immitis; Dog; Meshkin-Shahr; Molecular analysis
Subjects:QX Parasitology
ID Code:8844
Deposited By: خانم زهرا حیدری سورشجانی
Deposited On:01 Oct 1397 16:34
Last Modified:01 Oct 1397 16:34

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