لیشمانیازیس احشایی سگ سانان در سگ سانان وحشی (روباه، شغال و گرگ) در شمال شرق ایران با استفاده از روش های پارازیتولوژی، سرولوژی و مولکولی

محبعلی, مهدی and ارزمانی, کوروش and زارعی, ذبیح اله and آخوندی, بهناز and حجاران, هما and رائقی, صابر and حیدری, زهرا and متولی حقی, سید موسی and الیکایی, سمیرا and موسی زاده مجرد, احمد and کاکوئی, زهرا (1395) لیشمانیازیس احشایی سگ سانان در سگ سانان وحشی (روباه، شغال و گرگ) در شمال شرق ایران با استفاده از روش های پارازیتولوژی، سرولوژی و مولکولی. Journal of Arthropod-Borne Diseases ــ 10 (4). pp. 538-545. شاپا 2322-2271


Official URL: http://jad.tums.ac.ir/index.php/jad/article/view/3...


Canine Visceral Leishmaniasis in Wild Canines (Fox, Jackal, and Wolf) in Northeastern Iran Using Parasitological, Serological, and Molecular Methods

English Abstract

Background: Although many studies had been conducted on various aspects of canine visceral leishmaniasis (CVL) in domestic dogs in the endemic areas of Iran, investigations on CVL in wild canines are rare. Methods: This is a cross-sectional study was conducted from December 2012 to 2013 in northeast of Iran where human VL is endemic. Wild canines were trapped around the areas where human VL cases had been previously identified. Wild canines were collected and examined both clinically and serologically using direct agglutination test (DAT). Microscopically examinations were performed in all the seropositive wild canines for the presence of the amastigote form of Leishmania spp. Some Leishmania sp. which had been isolated from the spleens of wild canines, were examined analyzed by conventional PCR and sequencing techniques using α-tubulin and GAPDH genes. Results: Altogether, 84 wild canines including foxes (Vulpes vulpes, n=21), Jackals (Canis aureus, n=60) and wolves (Canis lupus, n=3) were collected. Four foxes and seven jackals showed anti-Leishmania infantum antibodies with titers of 1:320–1:20480 in DAT. Furthermore, one fox and one jackal were parasitologically (microscopy and culture) positive and L. infantum was confirmed by sequence analysis. Conclusion: The present study showed that sylvatic cycle of L. infantum had been established in the studied endemic areas of VL in northeastern Iran.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مهدی محبعلی
نویسنده :کوروش ارزمانی
نویسنده مسئول :ذبیح اله زارعی
نویسنده :بهناز آخوندی
نویسنده :هما حجاران
نویسنده :صابر رائقی
نویسنده :زهرا حیدری
نویسنده :سید موسی متولی حقی
نویسنده :سمیرا الیکایی
نویسنده :احمد موسی زاده مجرد
نویسنده :زهرا کاکوئی
Additional Information:Impact Factor (2016) 1.218 Indexed in: ISI, Scopus, Embase
کلیدواژه ها (انگلیسی):Canine visceral leishmaniasis, Wild canines, Iran
Subjects:QW Microbiology and Immunology
QX Parasitology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:8849
Deposited By: Dr Zahra Heidari
Deposited On:24 May 1396 09:28
Last Modified:14 Oct 1400 08:44

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