مقايسه اثر بخشی دو روش فوتودینامیک تراپی توسط نیو متیلن بلو و ایندوسیانین سبز ضد میکروبی بر روی تریکوفیتون روبروم درمحیط آزمایشگاهی

فکرآزاد, رضا and پورستاربجه میر, آرش and کهیایی اقدم, مینا and قاسمی برقی, ودود (1396) مقايسه اثر بخشی دو روش فوتودینامیک تراپی توسط نیو متیلن بلو و ایندوسیانین سبز ضد میکروبی بر روی تریکوفیتون روبروم درمحیط آزمایشگاهی. Photodiagnosis and Photodynamic Therapy ــ 18 . pp. 208-212. شاپا 1572-1000


Official URL: http://www.sciencedirect.com/science/journal/15721...


Comparison of photoinactivation of T. rubrum by new methylene blue(NMB) and indocyanine green (EmunDo®)

English Abstract

Background: Superficial mycotic skin infections which are predominantly caused by Trichophyton rubrum,poorly responsd to conventional therapies. A great amount of attention has focused on finding moreeffective treatments. The current work is aimed to compare the effectiveness of phoinactivation of Tri-chophyton rubrum by two relatively new photosensitizers: a phenothiazinium dye(New methylene blue)and Indocyanine green (EmunDo®).Materials and methods: A Final inoculum of T. rubrum which corresponded to 106colony forming unitper milliliter (CFU ml−1) was prepared. Antimicrobial Photodynamic treatment (aPDT) of T. rubrum wascarried out by either EmunDo®(1 mg/ml, Infra-red laser (IRL, � = 810 nm, Energy Density 55 J/cm2)) orNMB (10 �M, Red laser (RL), � = 630 nm, Energy Density of 5 J/cm2). The suspensions thereafter weresubcultured on Sabouraud dextrose agar (SDA) and were counted on due time. based on colony-formingunit per milliliter (CFU/ml).Results: aPDT with either EmunDo®(E) or NMB (N) considerably diminished the viability of inoculatedT. rubrum with respective reduction of 0.64 log and 0.4 log compared to the control group (P < 0.001). Nosignificant difference was found between two laser only groups (P = 0.79) and two aPDT groups (P = 0.73),however significant reduction of T. rubrum in red laser only group (P = 0.04) and EmunDo®only group(P = 0.04) was found as compared to the control group (P < 0.05).Conclusion: The study provides evidence regarding satisfactory photodynamic inactivation of T. rubrumwith EmunDo®or NMB as photosensitizers. Irradiation by only red laser source was found superior toonly infra-red laser source. Dark toxicity of EmunDo®was more successful than new methylene bluedye.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :رضا فکرآزاد
نویسنده :آرش پورستاربجه میر
نویسنده مسئول :مینا کهیایی اقدم
نویسنده :ودود قاسمی برقی
Additional Information:Impact Factor(2016) 2.219 Indexed in: ISI, Pubmed/ Medline/Index Medicus, Scopus, National Library of Medicine, ScienceDirect
کلیدواژه ها (انگلیسی):Photoinactivation, Photosensitizer, Trichophyton rubruma
Subjects:WR Dermatology
Divisions:Department of Dentistry > Department of Oral Surgery, and Maxillofacial
ID Code:8927
Deposited By: Dr Vadoud Ghasemi barghi
Deposited On:03 Apr 1396 10:00
Last Modified:03 Apr 1396 10:00

Repository Staff Only: item control page

Document Downloads

More statistics for this item...