ترکیب اثر آنتی اکسیدانی اریتروپویتین و ملاتونین بر آسیب کلیه ناشی از ایسکمی-رپرفیوژن در موشهای صحرائی نر

Title

Combination antioxidant effect of erythropoietin and melatonin on renal ischemia reperfusion damage in male rats

English Abstract

Ischemia reperfusion (IR) contributes to acute renal failure (ARF). Oxygen free radicals are involved in pathophysiology of ischemia reperfusion injury (IRI). This study was designed to investigate the effects of erythropoietin (EPO) and melatonin (MEL), which are known antiinflammatory and antioxidant agents, in IR-induced renal injury in rats. Male Wistar Albino rats were unilaterally nephrectomized and subjected to 45 min of renal pedicle occlusion followed by 24 h of reperfusion. MEL (10mg/kg, i.p) and EPO (5000U/kg, i.p) were administered prior to ischemia. After 24 h of the reperfusion, kidneys were removed and weighted, blood samples were collected for the determination of total antioxidant capacity (TAC), cholesterol and uric acid levels. The level of malondialdehyde (MDA) was measured in renal samples. Treatment with EPO or MEL had a curative effect on renal IR injury. The EPO+MEL group showed significantly greater improvement on renal weight and TAC levels when compared with the other treatment groups, moreover, this group significantly decreased MDA, cholesterol and uric acid levels. Our results indicate that the combination of erythropoietin and melatonin may exert more beneficial effects than either agent used alone

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