سلول های اپی/پرینورال، سلول های شوآن و همچنین یکپارچگی غلاف پرینورال به دنبال مواجهه با 2,4-D تحت تاثیر قرار می گیرند

شریفی پاسندی, مرضیه and حسینی شیرازی, فرشاد and غلامی, محمد رضا and صالحی, حسین and نجف زاده, نوروز and مأذنی, محمد and قاسمی حمیدآبادی, هاتف and نیاپور, علی (1396) سلول های اپی/پرینورال، سلول های شوآن و همچنین یکپارچگی غلاف پرینورال به دنبال مواجهه با 2,4-D تحت تاثیر قرار می گیرند. Neurotoxicity Research ــ 32 (4). pp. 624-638. شاپا 1029-8428

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Official URL: https://link.springer.com/article/10.1007%2Fs12640...


Epi/perineural and Schwann Cells as Well as Perineural Sheath Integrity are Affected Following 2,4-D Exposure

English Abstract

2,4dicholorophenoxy acetic acid (2,4-D) is a worldwide-known hormone herbicide. However, there are increasing concerns about its exposure and risks of developing pathological conditions for the peripheral nervous system. The aim of this study was to investigate the mechanism(s) involved in the toxicity of 2,4-D on peripheral nerve's cellular components. The epi/perineural and Schwann cells and a total of three cell lines were treated with 2,4-D. The viability of cells at different doses of 2,4-D was measured by MTT assay. The cell cycle analyses, cumulative cell counting, fluorescent staining, antioxidant and caspase enzymes activity were examined on epi/perineural and Schwann cells. The epi/perineural cells were assessed as having biological macromolecular changes. Some tight junction-related genes and proteins were also tested on explants of 2,4-D treated epi/perineural tissue. The viability of 2,4-D treated cells was reduced in a dose-dependent manner. Reduced growth rate and G1 cell cycle arrest were verified in 2,4-D treated epi/perineural and Schwann cells. The use of staining methods (acridine orange/ethidium bromide and DAPI) and caspase 3/7 activity assay along with malondialdehyde, glutathione peroxidase, and superoxide dismutase activity assays indicated the apoptotic and oxidant effects of 2,4-D on epi/perineural and Schwann cells. Data obtained from FTIR revealed changes in epi/perineural proteins and cell membrane lipids. Additionally, claudin-1, occludin, and ZO-1 gene/protein expression profiles were significantly reduced in 2,4-D-treated epi/perineural pieces. Our data indicated that oxidative stress, apoptosis of epi/perineural and Schwann cell and impaired blood-nerve barrier may have contributed to nerve damage following 2,4-D exposure.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :مرضیه شریفی پاسندی
نویسنده :فرشاد حسینی شیرازی
نویسنده :محمد رضا غلامی
نویسنده :حسین صالحی
نویسنده :نوروز نجف زاده
نویسنده :محمد مأذنی
نویسنده :هاتف قاسمی حمیدآبادی
نویسنده مسئول :علی نیاپور
Additional Information:IF: 3.311 Indexed in: ISI, PubMed/Medline, Scopus, EMBASE,
کلیدواژه ها (انگلیسی):Epi/perineural cells , Schwann cells , 2,4-D , Apoptosis , Claudin-1 , ZO-1
Subjects:QV pharmacology > QV 600 Toxicology
QS Human Anatomy
QU Biochemistry
QV pharmacology
QY Clinical Pathology
WL Nervous system
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Biochemistry
Faculty of Medicine > Department of Basic Sciences > Department of Anatomy
ID Code:9044
Deposited By: Dr Ali Niapour
Deposited On:26 May 1396 05:30
Last Modified:29 May 1401 10:08

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