بررسی فیلوژنتیک مولکولی گونه های تریکواسترونژیلوس (Nematoda: Trichostrongylidae) از انسان در استان مازندران، ایران

شریف دینی, میثم and حیدری, زهرا and حصاری, زهرا and وطن دوست, سجاد and کیا, عشرت بیگم (1396) بررسی فیلوژنتیک مولکولی گونه های تریکواسترونژیلوس (Nematoda: Trichostrongylidae) از انسان در استان مازندران، ایران. The Korean Journal of Parasitology ــ 55 (3). pp. 285-279. شاپا 0023-4001


Official URL: http://parasitol.kr/journal/view.php?number=2135


Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran

English Abstract

The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0–1.8% and 0–0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.

Item Type:Article
زبان سند : انگلیسی
نویسنده اول :میثم شریف دینی
نویسنده :زهرا حیدری
نویسنده مسئول :عشرت بیگم کیا
Additional Information:Impact Factor (2016) 0.889 Indexed in: ISI, Pubmed/Medline/Index Medicus, Scopus
کلیدواژه ها (انگلیسی):Trichostrongylus colubriformis, Trichostrongylus axei, human, PCR, ITS2-rDNA region, phylogenetic analysis, Mazandaran, Iran
Subjects:QX Parasitology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:9054
Deposited By: Dr Zahra Heidari
Deposited On:08 Jun 1396 05:55
Last Modified:14 Oct 1400 08:39

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