title

بررسی مورفولوژیکی و مولکولی دیکتیوکالوس فیلاریا جدا شده از گوسفند

حیدری, زهرا and موبدی, ایرج and میرهندی, حسین and میکائیلی, فتانه and زارعی, ذبیح الله and کیا, عشرت بیگم (1391) بررسی مورفولوژیکی و مولکولی دیکتیوکالوس فیلاریا جدا شده از گوسفند. در: 1st International and 8th National Congress of Parasitology and Parasitic Diseases in Iran, Oct 17-19, 2012, Kerman - Iran.

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Title

Morphological and molecular study on Dictyocaulus filaria isolated from sheep

English Abstract

The nematode Dictyocaulus filaria is a lungworm in small ruminant. In this study, morphological and molecular characterization of the worms isolated from sheep in Meshkinshar, Ardabil Province, Iran were studied. Two isolates of D. filaria were found among 90 respiratory tract samples of sheeps. Morphological features of worms were studied using a microscope equipped with camera lucida drawing tube. For molecular analysis, genomic DNA was extracted, PCR amplified, ITS-2 region was sequenced, aligned and compared with published sequences in GenBank. Morphologically, both isolates were similar, and their ITS-2 gene sequences were also completely identical. Analysis of the PCR products revealed that the samples are highly homologous, having 100% identity with D.filaria available in GenBank (accession number AJ580770.1). We found two isolate of D.filaria verified by sequence analyse of ITS-2 region.

Item Type:Conference or Workshop Item (Poster)
زبان سند : انگلیسی
نویسنده اول :زهرا حیدری
Subjects:QX Parasitology
Divisions:Faculty of Medicine > Department of Basic Sciences > Department of Parasitology
ID Code:9071
Deposited By: خانم زهرا حیدری سورشجانی
Deposited On:25 Jul 1397 10:11
Last Modified:25 Jul 1397 10:11

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