همزیستی کرم بالغ، لارو و تخم سیستوکالوس اوکراتوس (Cystocaulus ocreatus) با پروتواسکولکس های کیست هیداتیک ریوی گوسفند

عنوان انگليسی

Coexistence of adult worms, larvae and eggs of Cystocaulus ocreatus with hydatid cysts protoscoleces in sheep lungs

خلاصه انگلیسی

Cystocaulus ocreatus is a small lung worm that lives in the lung parenchyma and in subpleural nodules of small ruminants. The larval stage of Echinococcus granulosus or hydatid cyst affect rumminant 's lung,in addition to other tissues.In this study, coexistence of these two parasites in sheep is reported. In 2010, 90 hydatid cyst from sheep 's lung were collected from a traditional slaughterhouse in Meshkinshahr, Ardabil province, Iran. During examination of the cysts, in two cases coexistance of a lung nematode with hydatid cyst protoscolices was found. Morphological and morphpmetric characterisations of the nematode were verified. Genomic DNA was extracted from larva and adult. Using PCR, ITS2-rDNA region were amplified; the PCR products were sequenced and the results compared with the sequences in GenBank. Both isolates were identified as C. ocreatus based on morphological features and the sequence of the nuclear ITS2 region. In one case, only alive C. ocreatus larvae and in the second case, alive egges, larvae and adult male and female were coexisted with protoscolices. Sequences of both isolate (KJ152179.1, KJ152178.1) were homologe, with 100% similarity with registered sequences of C. ocreatus in GenBank.Coexistence of alive eggs, larvae and adults of C. ocreatus with hydatid cyst protoscolices is a rare and interesting phenomenon. Apparently, chemical composition of hydatid cyst fluid is a suitable medium for development of C. ocreatus.

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